Expression and detection significance of CARDS toxin in bronchoalveolar lavage fluid in children with severe Mycoplasma pneumoniae pneumonia

doi:10.3969/j.issn.1006-7795.2025.02.020

Abstract

Abstract: Objective To analyze the expression and detection significance of community acquired respiratory distress syndrome (CARDS) toxin in bronchoalveolar lavage fluid (BALF) in children with severe Mycoplasma pneumoniae pneumonia (SMPP). Methods The BALF samples of children diagnosed with Mycoplasma pneumoniae pneumonia (MPP) were collected from the Department of Pulmonology， Children's Hospital Affiliated to Capital Institute of Pediatrics from August 2021 to April 2022, the relative expression of CARDS toxins was determined by quantitative realtime polymerase chain reaction (qPCR). The patients were divided into SMPP group and non-SMPP group (control group). The relative expression of CARDS toxin and other clinical indicators were compared between the two groups. The risk factors of SMPP were analyzed. The predictive value of the indicators was analyzed using receiver operating characteristic (ROC) curves. Results ①The relative expression level of CARDS toxin (Z=－6.151), D-dimer (Z=－5.102) and lactate dehydrogenase (LDH) (Z=－6.337) in SMPP group were significantly higher than those in control group (P<0.001). The white blood cell (Z=－2.155), neutrophil ratio (t=2.988) and C-reactive protein (CRP) (Z=－3.360) were higher than those in control group (P<0.05). ②The relative expression of CARDS toxin was positively correlated with serum LDH (r=0.258, P<0.05). ③Binary Logistic regression analysis showed that fever time (OR=1.271, 95%CI:1.079-1.497), LDH (OR=1.011, 95%CI:1.005-1.017) and CARDS toxin (OR=6.210, 95%CI: 2.646-14.577) were risk factors for SMPP. Conclusion CARDS toxin was closely related to MPP disease, and was significantly increased in SMPP group. CARDS toxin was one of the independent risk factors for SMPP and positively correlated with serum LDH level. It can be used as a good indicator to evaluate the condition，combined with fever time and LDH has clinical significance.

Key words: Mycoplasma pneumoniae pneumonia, community acquired respiratory distress syndrome toxin, children, bronchoalveolar lavage fluid, risk factor

CLC Number:

R563.1

Zheng Baoying, Fu Hanyu, Xue Guanhua, Cao Ling. Expression and detection significance of CARDS toxin in bronchoalveolar lavage fluid in children with severe Mycoplasma pneumoniae pneumonia[J]. Journal of Capital Medical University, 2025, 46(2): 333-339.

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