Effects of Jieduan Niwan Formula on the c-Jun,p-JNK of liver tissue in Acute-on-Chronic liver failure rats

doi:10.3969/j.issn.1006-7795.2017.02.023

Abstract

Abstract: Objective To observe the effect of the Jieduan-Niwan Formula (JDNW)on the serum tumor necrosis factor alpha(TNF-α)content,the expression of p-JNK and transcription factor c-Jun in acute-on-chronic liver failure(ACLF)rats,to discuss part of the action mechanism of intervention of in ACLF. Methods SPF 70 male Wistar rats were randomly divided into normal control group,model group,JDNW group and JNK inhibitor SP600125 group,to inject pig serum to induce rat liver fibrosis model,and then acute attack combined with D-GalN/LPS,ACLF model was established. Rats in JDNW group were orally given JDNW formula for 3 days before acute attack.Rats in SP600125 group giving intraperitoneal injection half an hour before acute attack.Rats were sacrificed, respectively at 4,8 and 12 h after model established.The expression of TNF-α,c-Jun,p-JNK were detected by enzyme-linked immune sorbent assay(ELISA),western blot method and immunohistochemical analysis, respectively. Results Compared with normal group,the expression of TNF-α,p-JNK,c-Jun of model group were gradually increased;compared with the model group,the expression of TNF-α,p-JNK,c-Jun of JDNW and SP600125 group in 4h and 8h was less(P<0.05),and the expression of 12h increased; the different expression of SP600125 group was in accordance with the JDNW group. Conclusion The prescription of JDNWcan reduce the damage of rat liver cell ACLF in a certain extent,its mechanism may be through cell apoptosis mediated by JNK signaling pathway,thereby protecting liver cells,inhibiting liver failure.

Key words: acute-on-chronic liver failure, Jieduan Niwan Formula, apoptosis, JNK signaling pathway

CLC Number:

R285.6

Mu Lingyun, Li Jinxia, Zhang Qiuyun, Chen Yu, Gao Lianyin, Du Yuqiong. Effects of Jieduan Niwan Formula on the c-Jun,p-JNK of liver tissue in Acute-on-Chronic liver failure rats[J]. Journal of Capital Medical University, 2017, 38(2): 282-288.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.02.023

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/282

References

[1] 中华医学会感染病学分会肝衰竭与人工肝学组,中华医学会肝病学分会重型肝病与人工肝学组.肝衰竭诊疗指南(2012年版)[J].实用肝脏病杂志,2013,16(3):210-216.
[2] 刘增利,张秋云.截断逆挽方对慢加急性肝衰竭大鼠ET、TNF-α的影响[J].中国医药导刊,2011,13(4):650-652.
[3] 吴文秀,崔利娟,陈煜,等.截断逆挽方对慢加急性肝衰竭大鼠肝细胞凋亡指数及caspase8、caspase3表达的影响[J].北京中医药,2012,32(1):62-64.
[4] 吴文秀,崔利娟,张秋云.截断逆挽方降低慢加急性肝衰竭大鼠死亡率的机制探讨[J].中医药导报,2012,18(1):6-11.
[5] 崔利娟,党泽方,张秋云.截断逆挽方对慢加急性肝衰竭大鼠血清TNF-α、IL-1β、IL-6的影响[J].中医药导报,2013,19(1):8-11.
[6] 崔利娟,吴文秀,邢金丽,等.截断逆挽方对慢加急性肝衰竭大鼠血清及肝组织TNF-α含量及肝组织TNFR1表达的影响[J].北京中医药,2013,32(2):134-138.
[7] 党泽方,李金霞,张秋云,等.截断逆挽方对慢加急性肝衰竭大鼠肝细胞超微结构、周期蛋白Cyclin E及转录因子DP1的影响[J].河北医药,2015,37(9):1285-1289.
[8] 李金霞,穆凌云,张秋云,等.截断逆挽方对慢加急性肝衰竭大鼠线粒体凋亡途径的影响[J].中国中医药信息杂志,2016,23(4):45-48.
[9] 刘旭华,孟艳英,陈煜,等.采用免疫型肝硬化大鼠建立慢加急性肝衰竭模型方法的研究[J].胃肠病学和肝病学杂志,2008,17(10):790-793.
[10] 中国中西医结合学会肝病专业委员会.肝纤维化中西医结合诊疗指南[J].中华肝脏病杂志,2006,14(11):866-870.
[11] Olson J C, Wendon J A, Kramer D J, et al. Intensive care of the patient with cirrhosis[J].Hepatology, 2011,54:1864-1872.
[12] 曾庆磊,吕君,等.慢加急性肝衰竭的定义与治疗[J].肝脏,2016,21(1):66-67.
[13] 吴人亮,李娜萍.细胞凋亡、胀亡和坏死———关于细胞死亡的新认识[J].中华病理学杂志,2002,31(5):455-456.
[14] 王漫.JNK抑制剂SP600125对AD脑内突触损害和炎性反应治疗作用及其机制研究[D].西安:第四军医大学,2015.
[15] Lai B,Pu H,Cao Q,et al.Activation of caspase-3 and c-Jun NH2-terminal kinase signaling pathways involving heroin-induced neuronal apoptosis[J].Neurosci Lett,2011,502(3):209-213.