Human ether-α-go-go related gene channels improves glucose metabolism via anti-oxidative stress in HepG2 cells

doi:10.3969/j.issn.1006-7795.2017.02.003

Abstract

Abstract: Objective To evaluate the effect of the new pathway of human ether-α-go-go related gene(hERG) channels on glucose metabolism in hepatic cells. Methods Liver hepatocellular carcinoma (HepG2) cells were transfected with hERG channels over-expression plasmid DNA. pcDNA3. 1-GFP plasmid was used as a negative control. The expression of hERG, phosphoenolpyruvate carboxykinase(PEPCK) and glucose-6-phosphatase (G6Pase) were detected by Western blotting. The mRNA level of glucose transporter type 2 (GLUT2), insulin receptor substrate (IRS-2), adenosine 5'-monophosphate(AMP)-activated protein kinase (AMPKα2), p22, p47, p67 and p91 were observed by real-time PCR(RT-PCR). Results The experiments showed that hERG gene can be expressed in HepG2 cells. Over-expression of hERG gene in HepG2 cells could down-regulate the expression of PEPCK, G6Pase. while GLUT2, IRS-2, AMPKα2 genes were up-regulated. Over-expression of hERG gene also inhibited the relative mRNA level of p22, p47, p67and p91. Conclusion hERG channels could be involved in improving glucose metabolism via anti-oxidative effects.

Key words: human ether-α-go-go related gene channels, insulin resistance, oxidative stress, glucose metabolism

CLC Number:

R587.1

Lu Jing, Sheng Han, Cheng Cheng, Song Lini, Zhang Yichen, Xie Rongrong, Yuan Mingxia, Yang Jinkui. Human ether-α-go-go related gene channels improves glucose metabolism via anti-oxidative stress in HepG2 cells[J]. Journal of Capital Medical University, 2017, 38(2): 156-160.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.02.003

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/156

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