Oxidative stress mediates the effect of NLRP3 inflammasome on chronic obstructive pulmonary disease

doi:10.3969/j.issn.1006-7795.2020.01.025

Abstract

Abstract: Objective The correlation among nucleotide binding oligomerization domain like receptor protein 3(NLRP3), malondialdehyde(MDA), superoxide dismutase(SOD) and forced expiratory volume in the first second% pred(FEV1% pred) was analyzed by detecting the levels of SOD, MDA and the expression level of NLRP3 mRNA in serum of COPD patients, so as to explore the influence of oxidative stress-mediated NLRP3 inflammatory body activation on COPD. Methods Selection of Changsha's Hospitals affiliated to Nanhua University in June 2017 to June 2018, 90 cases of COPD patients including 60 patients with acute chronic obstructive pulmonary disease (AECOPD).According to pulmonary function in patients with AECOPD group is divided into weight very restructuring and light-medium group, 30 cases in each group.Selected 30 COPD patients who reached the level remission after treatment symptoms and selected the other 30 cases who got healthy subjects through physical examination as a control group.Serum MDA, SOD levels and NLRP3 mRNA expression levels in AECOPD group, COPD stable period group, control group were compared. Otherwise, serum MDA, SOD levels and NLRP3 mRNA expression levels in weight-very restructuring group, light-medium group and healthy control group were compared.Pearson correlation analysis was used to analyze the correlation between serum MDA and SOD levels, NLRP3 mRNA expression in peripheral blood lymphocytes, and FEV1% pred in COPD patients.Results Serum MDA level in AECOPD group was higher than that in COPD stable period group and COPD stable period group was higher than that in healthy control group (P<0.05). Serum SOD level in AECOPD group was lower than that in COPD stable period group and COPD stable period group was lower than that in healthy control group (P<0.05).The expression level of NLRP3 mRNA in peripheral blood lymphocytes in AECOPD group was higher than that in COPD stable period group and COPD stable period group was higher than that in healthy control group (P<0.05). Serum MDA level in the weight-very restructuring group was higher than that in the light-medium group and the light-medium group was higher than that in the healthy control group (P<0.05). Serum MDA level in the weight-very restructuring group was lower than that in light-medium group and light-medium group was lower than that in the healthy control group (P<0.05). The expression level of NLRP3 mRNA in peripheral blood lymphocytes in weight-very restructuring group was significantly higher than that in the light-medium group and the light-medium group was higher than that in the healthy control group (P<0.05). The result of pearson correlation analysis showed that serum MDA level in COPD patients was positively correlated with NLRP3 mRNA expression, with a correlation coefficient r of 0.64(P<0.05). Serum SOD level was negatively correlated with NLRP3 mRNA expression in COPD patients, with a correlation coefficient r of -0.90(P<0.05). In COPD patients, FEV1%pred was negatively correlated with NLRP3 mRNA expression, and the correlation coefficient r was -0.78 (P<0.05). Conclusion Oxidative stress and NLRP3 inflammatory corpuscle are involved in the pathogenesis of COPD. The increasing of oxidative stress and NLRP3 mRNA expression were correlated with exacerbations of COPD. Oxidative stress-mediated activation of NLRP3 inflammatory corpuscle has an impact on the progression of COPD.

Key words: oxidative stress, NLRP3 inflammasome, chronic obstructive pulmonary disease(COPD)

CLC Number:

R681

Liu Ping, Lou Ke, Weng Long, Li Hui, Tang Yuling. Oxidative stress mediates the effect of NLRP3 inflammasome on chronic obstructive pulmonary disease[J]. Journal of Capital Medical University, 2020, 41(1): 131-136.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.01.025

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I1/131

References

[1] 陈亚红. 2017年GOLD慢性阻塞性肺疾病诊断、治疗及预防的全球策略解读[J].中国医学前沿杂志(电子版),2017,9(1):37-47.
[2] Ting J P Y,Lovering R C,Alnemri E S,et al. The NLR gene family:an official nomenclature[J]. Immunity,2008,28(3):285.
[3] Cassel S L,Joly S,Sutterwala F S. The NLRP3 inflammasome:a sensor of immune danger signals[J]. Semin Immunol,2009,21(4):194-198.
[4] Schroder K,Tschopp J. The inflammasomes[J]. Cell,2010,140(6):821-832.
[5] Bartoli M L,Novelli F,Costa F,et al. Malondialdehyde in exhaled breath condensate as a marker of oxidative stress in different pulmonary diseases[J]. Mediat inflam,2011,(4):891752.
[6] Ismail M,Hossain M F,Tanu A R,et al. Effect of spirulina intervention on oxidative stress,antioxidant status,and lipid profile in chronic obstructive pulmonary disease patients[J]. Biomed Res Int,2015,2015:1-7.
[7] Zeng M,Li Y,Jiang Y,et al. Local and systemic oxidative stress status in chronic obstructive pulmonary disease patients[J]. Canadian Respir J,2013,20(1):35.
[8] Ben Anes A,Fetoui H,Bchir S,et al. Increased oxidative stress and altered levels of nitric oxide and peroxynitrite in Tunisian patients with chronic obstructive pulmonary disease:correlation with disease severity and airflow obstruction[J]. Biol Trace Element Res,2014,161(1):20-31.
[9] Antus B,Harnasi G,Drozdovszky O,et al. Monitoring oxidative stress during chronic obstructive pulmonary disease exacerbations using malondialdehyde[J]. Respirology,2014,19(1):74-79.
[10] 樊涛,张宇,蒋洪丽,等.慢性阻塞性肺疾病患者肺组织丙二醛、白细胞介素8、肿瘤坏死因子α水平及吸烟的影响[J].中国呼吸与危重监护杂志,2012,11(3):218-222.
[11] 蒋雪莲,钟萍,黄成亮,等.氧化应激反应标志物在慢性阻塞性肺疾病稳定期患者血清中的表达及意义[J].中国呼吸与危重监护杂志,2016(6):542-547.
[12] Arif E,Vibhuti A,Deepak D,et al. COX₂ and p53 risk-alleles coexist in COPD[J]. Clin Chim Acta,2008,397(1-2):0-50.
[13] Keranis E,Makris D,Rodopoulou P,et al. Impact of dietary shift to higher-antioxidant foods in COPD:a randomised trial[J]. Eur Respir J,2010,36(4):774-780.
[14] 毛建,宋珊,贾钦尧,等.慢性阻塞性肺疾病患者外周血淋巴细胞NLRP3 mRNA的表达及血清IL-1β、IL-18检测的意义[J].临床肺科杂志,2017(6):98-101.
[15] 王艳蕾.氧化/抗氧化失衡与COPD急性加重期炎症反应的关系[J].天津医药,2012,41(4):327-329.
[16] 杜强,朱成华,沈立,等.慢性阻塞性肺疾病患者SIRT1表达与氧化应激的相关性研究[J].临床肺科杂志,2016,21(7):1213-1215.
[17] 王袁,戈艳蕾,刘聪辉,等.支气管炎型与肺气肿型AECOPD患者血浆periostin、bFGF、TNF-α、IL-6、IL-8水平变化[J].中国煤炭工业医学杂志,2018,21(5):478-482.
[18] Drefs M, Thomas M N, Guba M, et al. Modulation of glutathione hemostasis by inhibition of 12/15-lipoxygenase prevents ROS-mediated cell death after hepatic ischemia and reperfusion[J]. Oxid Med Cell Longev, 2017,2017:8325754.
[19] Chen L, Liu P, Feng X, Ma C. Salidroside suppressing LPS-induced myocardial injury by inhibiting ROS-mediated PI3K/Akt/mTOR pathway in vitro and in vivo[J]. J Cell Mol Med,2017,21(12):3178-3189.