Significance of NADPH oxidases NOX1, NOX2 and DUOX2 expression in inflammatory bowel disease

doi:10.3969/j.issn.1006-7795.2017.02.014

Abstract

Abstract: Objective To investigate the expression of NOX1, NOX2 and DUOX2, the members of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase family, in large intestine of patients with inflammatory bowel disease (IBD) and mouse colitis and analyze the relationship between these enzymes and IBD. Methods The samples were collected from large intestine of patients with IBD. Mouse chronic colitis model was established by using eight to ten-week-old C57BL/6 mice treated with three cycles of drinking 1.0% dextran sulfate sodium (DSS) for 7 days plus drinking water for 14 days. Mouse body weight change and hematoxylin-eosin (HE) staining of colon sections were used to evaluate the severity of inflammatory lesions. The expression of NOX1, NOX2 and DUOX2 gene and protein were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. Results The expression of NOX1, NOX2 and DUOX2 mRNA significantly increased in human inflamed tissues compared to paired normal tissues (all P<0.01). NOX2 mRNA higher in male patients than female. Unlike human, only Nox2 mRNA increased by 2.4 times in chronic colitis mice, Nox1 and Duox2 mRNA remained unchanged. These three proteins in IBD patients increased (all P<0.01); in chronic colitis mice Nox2 and Duox2 proteins increased (both P<0.01), but Nox1 did not. Conclusion NOX1, NOX2 and DUOX2 not only play an important role in maintaining the normal physiological function, but also were associated with early onset IBD.

Key words: NADPH oxidases, NOX1, NOX2, DUOX2, inflammatory bowel disease (IBD)

CLC Number:

R574

Xuan Qingxia, Dai Faliang, Chen Pan, Feng Dandan, Jin Jianjun, Gao Qiang. Significance of NADPH oxidases NOX1, NOX2 and DUOX2 expression in inflammatory bowel disease[J]. Journal of Capital Medical University, 2017, 38(2): 220-226.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.02.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/220

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