Study on the gastric myoelectric signal recording by implantable radiotelemetry in conscious rats with free movement

doi:10.3969/j.issn.1006-7795.2017.02.015

Abstract

Abstract: Objective To optimize the recording method on the gastric myoelectric activity, reduce operation trauma to animals and improve the animal survival rate after surgery. Methods The optimized recording electrodes were implanted into the serosal layer of gastric antrum. 7 days after surgery, the gastric myoelectricity signals were collected from conscious and unrestrained rats through the implantable physiological signal wireless telemetry system. The frequency, amplitude and characteristics of gastric electromyography in digestive and interdigestive phase were analyzed. Results In digestive stage, sustainable and stable gastric myoelectricity signals were recorded. The amplitude and frequency of gastric slow wave (SW) is (14.39±1.12)μV and (4.76±0.09)cpm, respectively; amplitude of gastric spike potentials (SP) is (70.76±5.31)μV and the mean number of SP on each SW is (10.38±1.22). In interdigestive phase, each cycle duration is 10-12 min, the third period of gastric migrating myoelectric/motor complex (MMC) is strongest. The amplitude and frequency of gastric SW is (14.75±0.76)μV and (4.90±0.14)cpm, respectively; amplitude of gastric SP is (72.58±3.67)μV, the mean number of SP on each SW is 12.00±1.35. In the digestion period, the use of prokinetic drug, domperidone (1mg/kg) increased the amplitude (16.87±1.05)μV to (25.77±2.16)μV (P<0.01, n=6) and frequency (4.97±0.18) cmp to (4.85±0.14)cmp (P>0.05, n=6) of SW and amplitude (72.25±3.65)μV to (104.00±7.72)μV (P<0.01, n=6) and frequency (10.50±1.12) to (14.00±0.97) (P<0.05, n=6) of SP. Conclusion Using implantable radiotelemetry, we successfully acquired rat gastric myoelectricity signals in digestive and interdigestive phase under physiological state, which provided a more accurate and effective experimental technique/method for exploring gastric myoelectric activity and then the function of gastric motility under physiologic and pathophysiologic state.

Key words: implantable radiotelemetry system, gastric myoelectricity, slow wave and spike potentials, domperidone

CLC Number:

Yao Yuansheng, Zheng Lifei, Zhu Jinxia. Study on the gastric myoelectric signal recording by implantable radiotelemetry in conscious rats with free movement[J]. Journal of Capital Medical University, 2017, 38(2): 227-231.

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https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/227

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