Generation and identification of Unc13 gene pancreatic beta cell knockout mice model

doi:10.3969/j.issn.1006-7795.2020.01.003

Abstract

Abstract: Objective To construct Unc13 gene pancreatic beta cell knockout mice by the Cre recombinase system for the study of the role and mechanism of Unc13 in insulin secretion. Methods Unc13flox/flox transgenic mice were constructed by CRISPR/Cas9 technology and hybridized with pancreatic beta cell specific Cre recombinase (Ins2-cre)tool mice. The progeny genotype was identified with PCR and sequencing technology. The knock out (KO) and their wild type (WT) mice were used to measure body weight, glucose tolerance, and insulin secretion level. Results The pancreatic beta cell specific Unc13 gene conditional knockout mice (hereinafter referred to as Unc13 KO mice) were constructed. Further studies showed that Unc13 KO mice had impaired glucose tolerance and decreased first phase insulin secretion but no significant change in body weight compared with WT mice. Conclusion The Unc13 KO mice model was constructed, which provides a research platform for exploring the role of Unc13 gene in the development of diabetes.

Key words: gene knockout, Cre-LoxP recombinase system, insulin secretion, Unc13 gene

CLC Number:

R587

Li Qi, Lu Jing, Zhu Xiaorong, Xiong Fengran, Yang Jinkui. Generation and identification of Unc13 gene pancreatic beta cell knockout mice model[J]. Journal of Capital Medical University, 2020, 41(1): 14-20.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.01.003

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I1/14

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