Effects of storage condition on measurement of haptoglobin in urine

doi:10.3969/j.issn.1006-7795.2020.01.004

Journal of Capital Medical University ›› 2020, Vol. 41 ›› Issue (1): 21-26.doi: 10.3969/j.issn.1006-7795.2020.01.004

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Effects of storage condition on measurement of haptoglobin in urine

Zheng Xiaomin^1,2,3,4, Yang Fangyuan^2,3,4, Liu Cuiping¹, Yang Jinkui^2,3,4

1. Department of Endocrinology, Chuiyangliu Hospital affiliated to Tsinghua University, Beijing 100022, China;
2. Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
3. Beijing Key Laboratory of Diabetes Research and Care, Beijing 100730, China;
4. Beijing Diabetes Institute, Beijing 100730, China

Received:2019-12-12 Online:2020-02-21 Published:2020-02-13
Supported by:
This study was supported by National Key R&D Program of China(2017YFC0909600),National Natural Science Foundation of China(81561128015),Beijing Municipal Science & Technology Commission (D171100002817005).

Abstract

Abstract: Objective To investigate the effects of different treatments of urinary specimen and storage conditions on urinary haptoglobin levels. Methods The urinary samples of 40 patients with type 2 diabetes were stored at room temperature and 4℃ for 3 hours, 6 hours and 24 hours with and without centrifugation, and then the levels of urinary haptoglobin were measured with enzyme-linked immunosorbent assay. Results For the samples without centrifugation, no change in urinary haptoglobin concentration was observed after 3 hours and 6 hours of room temperature storage(both P>0.05), but there was a significantly decrease after 24 hours(P < 0.01). There was no statistically significant difference in the levels of urinary haptoglobin after 3 hours at 4℃ (P>0.05), and there was a significantly decrease after 6 hours(P < 0.01). The centrifuged samples were stable for 3 hours at both room temperature and 4℃(both P>0.05), but there was a significantly decrease after 6 hours(both P < 0.01).Conclusion It was recommended to store urine samples at room temperature when the measurement of urinary haptoglobin could be completed within 6 hours. Cold storage and centrifugation resulted in the decrease of urinary haptoglobin level.

Key words: haptoglobin, diabetic kidney disease, storage

CLC Number:

R587.2

Zheng Xiaomin, Yang Fangyuan, Liu Cuiping, Yang Jinkui. Effects of storage condition on measurement of haptoglobin in urine[J]. Journal of Capital Medical University, 2020, 41(1): 21-26.

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Effects of storage condition on measurement of haptoglobin in urine

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