Effect of shared peripheral circulation on renal injury in diabetic kidney disease mice

doi:10.3969/j.issn.1006-7795.2022.05.002

Journal of Capital Medical University ›› 2022, Vol. 43 ›› Issue (5): 674-679.doi: 10.3969/j.issn.1006-7795.2022.05.002

• Clinical and Basic Research in Nephrology • Previous Articles Next Articles

Effect of shared peripheral circulation on renal injury in diabetic kidney disease mice

Huang Qi¹, Zhou Yilun^1*, Sun Xuefeng^2*

1. Department of Nephrology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;
2. Department of Nephrology, Chinese People's Liberation Army General Hospital, State Key Laboratory of Kidney Disease, Beijing 100853, China

Received:2022-05-09 Online:2022-10-21 Published:2022-10-25
Contact: * E-mail:zhouyilun2008@sina.cn, xfssun@126.com
Supported by:
National Natural Science Foundation of China(81900662).

Abstract

Abstract: Objective To investigate the effects of the shared peripheral circulation on diabetic kidney disease (DKD).Methods Parabiotic surgery was performed between the 8-week-old male C57BL/6J mice and the DKD male mice of the same strain, the DKD mice was induced by streptozocin (STZ) injection and divided into three groups based on the stage of DKD: 12 weeks after STZ injection, 20 weeks after STZ injection, and 28 weeks after STZ injection. The animals were sacrificial 8 weeks after parabiotic surgery,and the blood, urine and kidney tissue samples of mice in each group were collected. Results A total of 24 pairs of parabiosis mice were obtained, 8 pairs in DKD 12 weeks, 8 pairs in DKD 20 weeks and 8 pairs in DKD 28 weeks. DKD mice in the same stage without peripheral circulation were set as the positive control group, and wild mice of the same age as the negative control group.The levels of blood glucose, insulin, triglyceride and total cholesterol in DKD with shared peripheral circulation group were significantly lower than those in the positive control group throughout all stage of DKD. In the DKD 20 weeks, the level of albumin-to-creatinine ratio in the group receiving shared peripheral circulation was significantly lower than that in the positive control group, with superiority was more significant in the DKD 28 weeks. In addition, in the DKD 28 weeks, the degree of renal injury and the expression of fibrosis marker protein in the positive control group were significantly higher than those in DKD mice receiving shared peripheral circulation. Conclusion Shared peripheral circulation could reduce blood glucose level, enhance insulin sensitivity and regulate lipid metabolism in DKD mice, and then alleviate renal tissue damage, improve renal fibrosis and protect renal function.

Key words: diabetic kidney disease, peripheral circulation, parabiosis animal model

CLC Number:

Huang Qi, Zhou Yilun, Sun Xuefeng. Effect of shared peripheral circulation on renal injury in diabetic kidney disease mice[J]. Journal of Capital Medical University, 2022, 43(5): 674-679.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2022.05.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2022/V43/I5/674

References

[1] Zhang L X, Long J Y, Jiang W S, et al. Trends in chronic kidney disease in China[J]. N Engl J Med, 2016, 375(9): 905-906.
[2] Gregg E W, Li Y F, Wang J, et al. Changes in diabetes-related complications in the United States, 1990-2010[J]. N Engl J Med, 2014, 370(16): 1514-1523.
[3] Wu T, Qiao S X, Shi C Z, et al. Metabolomics window into diabetic complications[J]. J Diabetes Investig, 2018, 9(2): 244-255.
[4] Huang Q, Ning Y C, Liu D, et al. A young blood environment decreases aging of senile mice kidneys[J]. J Gerontol A Biol Sci Med Sci, 2018, 73(4): 421-428.
[5] Liu D, Lun L D, Huang Q, et al. Youthful systemic milieu alleviates renal ischemia-reperfusion injury in elderly mice[J]. Kidney Int, 2018, 94(2): 268-279.
[6] Kamran P, Sereti K I, Zhao P, et al. Parabiosis in mice: a detailed protocol[J]. J Vis Exp, 2013(80): 50556.
[7] Loffredo F S, Steinhauser M L, Jay S M, et al. Growth differentiation factor 11 is a circulating factor that reverses age-related cardiac hypertrophy[J]. Cell, 2013, 153(4): 828-839.
[8] Villeda S A, Luo J, Mosher K I, et al. The ageing systemic milieu negatively regulates neurogenesis and cognitive function[J]. Nature, 2011, 477(7362): 90-94.
[9] Salpeter S J, Khalaileh A, Weinberg-Corem N, et al. Systemic regulation of the age-related decline of pancreatic β-cell replication[J]. Diabetes, 2013, 62(8): 2843-2848.
[10] Pietramaggiori G, Scherer S S, Alperovich M, et al. Improved cutaneous healing in diabetic mice exposed to healthy peripheral circulation[J]. J Invest Dermatol, 2009, 129(9): 2265-2274.
[11] da Silva A A, Hall J E, Dai X M, et al. Chronic antidiabetic actions of leptin: evidence from parabiosis studies for a CNS-derived circulating antidiabetic factor[J]. Diabetes, 2021, 70(10): 2264-2274.
[12] Boonloh K, Lee E S, Kim H M, et al. Rice bran protein hydrolysates attenuate diabetic nephropathy in diabetic animal model[J]. Eur J Nutr, 2018, 57(2): 761-772.
[13] Yu R C, Bo H, Villani V, et al. The inhibitory effect of rapamycin on toll like receptor 4 and interleukin 17 in the early stage of rat diabetic nephropathy[J]. Kidney Blood Press Res, 2016, 41(1): 55-69.
[14] Ducasa G M, Mitrofanova A, Fornoni A. Crosstalk between lipids and mitochondria in diabetic kidney disease[J]. Curr Diab Rep, 2019, 19(12): 144.
[15] Ducasa G M, Mitrofanova A, Mallela S K, et al. ATP-binding cassette A1 deficiency causes cardiolipin-driven mitochondrial dysfunction in podocytes[J]. J Clin Invest, 2019, 129(8): 3387-3400.

Effect of shared peripheral circulation on renal injury in diabetic kidney disease mice

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	Wang Mengdi, Guo Yifan, Pang Yanyu, Liu Yufei, Zhao Wenjing. Research of Baoshentongluo formula-medicated serum on regulating mitophagy and reducing oxidative damage of podocytes induced by high glucose [J]. Journal of Capital Medical University, 2022, 43(5): 687-693.
[2]	Zheng Xiaomin, Yang Fangyuan, Liu Cuiping, Yang Jinkui. Effects of storage condition on measurement of haptoglobin in urine [J]. Journal of Capital Medical University, 2020, 41(1): 21-26.
[3]	Zheng Xiaomin, Yang Fangyuan, Liu Cuiping, Yang Jinkui. Predictive value of haptoglobin on renal dysfunction in patients with early diabetic kidney disease [J]. Journal of Capital Medical University, 2019, 40(1): 53-58.