Effect of RFX6 on pancreas development in zebrafish using CRISPR/Cas9

doi:10.3969/j.issn.1006-7795.2015.06.002

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (6): 844-847.doi: 10.3969/j.issn.1006-7795.2015.06.002

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Effect of RFX6 on pancreas development in zebrafish using CRISPR/Cas9

Cheng Cheng^1,2, Wu Qian³, Lu Jing^1,2, Qiu Haiyan^1,2, Li Qian^1,2, Yang Jinkui^1,2

1. Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
2. Key Laboratory of Diabetes Prevention and Control, Beijing 100730, China;
3. Life Sciences, Peking University, Beijing 100871, China

Received:2015-10-21 Online:2015-12-21 Published:2015-12-18
Supported by:
This study was supported by National Natural Science Foundation of China(81270918, 81471014), Natural Science Foundation of Beijing(7131005).

Abstract

Abstract: Objective To construct the RFX6 knockout zebrafish animal model and preliminarily study the effects of RFX6 on the pancreatic development in zebrafish. Methods CRISPR/Cas9 technology was used to design gRNA against the target and induce mutation, thus the mutants can be obtained by sequencing. Results The RFX6 knockout zebrafish animal model was successfully constructed. Preliminary studies showed that RFX6 homozygous mutants suffered from growth retardation, lean and thin body with diabetes, and could not produce offsprings. Conclusion RFX6 plays an important role and is the key factor in the pancreatic development of zebrafish.

Key words: gene knockout, CRISPR/Cas9, zebrafish, pancreas development, RFX6

CLC Number:

R714.2

Cheng Cheng, Wu Qian, Lu Jing, Qiu Haiyan, Li Qian, Yang Jinkui. Effect of RFX6 on pancreas development in zebrafish using CRISPR/Cas9[J]. Journal of Capital Medical University, 2015, 36(6): 844-847.

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Effect of RFX6 on pancreas development in zebrafish using CRISPR/Cas9

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