Construction and preliminary application of BACE2 gene knockout in zebrafish using CRISPR/Cas9

doi:10.3969/j.issn.1006-7795.2015.06.001

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (6): 839-843.doi: 10.3969/j.issn.1006-7795.2015.06.001

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Construction and preliminary application of BACE2 gene knockout in zebrafish using CRISPR/Cas9

Lu Jing¹, Wu Qian², Xie Rongrong¹, Qiu Haiyan¹, Cheng Cheng¹, Yang Jinkui¹

1. Key Laboratory of Diabetes Prevention and Control, Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
2. Life Sciences, Peking University, Beijing 100871, China

Received:2015-10-21 Online:2015-12-21 Published:2015-12-18
Supported by:
This study was supported by National Natural Science Foundation of China(81270918, 81471014), Natural Science Foundation of Beijing(7131005).

Abstract

Abstract: Objective To construct β-site APP-cleaving enzyme 2 knockout zebrafish animal model and preliminarily explore its application. Methods By using the CRISPR/Cas9 technology, the gRNA against target in zebrafish was designed to induce mutations;afterwards the mutant was obtained by DNA sequencing. Results BACE2 homozygous mutants were unable to survive in the early part. Results of RT-PCR showed continued BACE2 gene expression in zebrafish early development. Conclusion BACE2 knockout zebrafish animal model may provide an effective tool for further study of the mechanism of pancreas development and drug screening.

Key words: gene knockout, CRISPR/Cas9, zebrafish, β-site APP-cleaving enzyme 2

CLC Number:

Lu Jing, Wu Qian, Xie Rongrong, Qiu Haiyan, Cheng Cheng, Yang Jinkui. Construction and preliminary application of BACE2 gene knockout in zebrafish using CRISPR/Cas9[J]. Journal of Capital Medical University, 2015, 36(6): 839-843.

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Construction and preliminary application of BACE2 gene knockout in zebrafish using CRISPR/Cas9

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