Phenotypic analysis of hepatocyte-specific Eva1a/Tmem166 gene knockout mice

doi:10.3969/j.issn.1006-7795.2021.04.010

Journal of Capital Medical University ›› 2021, Vol. 42 ›› Issue (4): 568-574.doi: 10.3969/j.issn.1006-7795.2021.04.010

• Basic Research • Previous Articles Next Articles

Phenotypic analysis of hepatocyte-specific Eva1a/Tmem166 gene knockout mice

Chen Jianhong¹, Lin Xin², Feng Jinqiu², Ding Pengpeng¹, Wang Miaomiao¹, Lin Lin¹, Liu Hong^1*, Wu Jing^3*

1. Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China;
2. Peking University Center for Human Diseases Genomics, Beijing 100083, China;
3. Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Diseases, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, Beijing 100050, China

Received:2020-12-01 Online:2021-08-21 Published:2021-07-29
Contact: * E-mail:wujing36youyi@ccmu.edu.cn; liuhong_sjt@ccmu.edu.cn
Supported by:
National Natural Science Foundation of China (81900505), Beijing Municipal Administration of Hospitals' Youth Programe (QML20200702), Youth Fund of Beijing Shijitan Hospital (2018-q09).

Abstract

Abstract: Objective To construct mice models with hepatocyte-specific knockout of autophagy related gene Eva1a using Cre-LoxP technique, and to preliminarily explore the effect of Eva1a gene knockout on the phenotype of mice. Methods LoxP-labeled Eva1a^flox/+ mice and Alb-Cre mice were used to construct homozygous mice models with hepatocyte-specific Eva1a gene knockout (Eva1a^flox/flox: Alb-Cre) through multiple generations of hybridization. Eva1a^flox/flox: Alb-Cre mice and Eva1aflox/flox mice were selected for phenotypic analysis, including body mass, liver index, liver histology, liver function, glycolipid metabolism, and autophagy level. Results There was no fetal death in homozygous Eva1a knockout mice, and no significant changes in the physiological condition were found. In conventional feeding conditions, hepatocyte-specific Eva1a knockout had no significant difference in body weight, liver appearance, tissue structure, liver index, liver function, glucose and lipid metabolism, and autophagy level from wild-type mice. Conclusion Hepatocyte-specific Eva1a gene knockout had no significant effect on the phenotype of mice in the physiological condition, and our study provided an animal model to further explore the role and molecular mechanism of Eva1a in liver disease.

Key words: Eva1a/Tmem166, gene knockout, liver, phenotype

CLC Number:

R575

Chen Jianhong, Lin Xin, Feng Jinqiu, Ding Pengpeng, Wang Miaomiao, Lin Lin, Liu Hong, Wu Jing. Phenotypic analysis of hepatocyte-specific Eva1a/Tmem166 gene knockout mice[J]. Journal of Capital Medical University, 2021, 42(4): 568-574.

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Phenotypic analysis of hepatocyte-specific Eva1a/Tmem166 gene knockout mice

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