Construction and preliminary application of conditional KCNH6-knockout mice

doi:10.3969/j.issn.1006-7795.2020.01.002

Journal of Capital Medical University ›› 2020, Vol. 41 ›› Issue (1): 8-13.doi: 10.3969/j.issn.1006-7795.2020.01.002

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Construction and preliminary application of conditional KCNH6-knockout mice

Lu Jing, Li Qi, Zhu Xiaorong, Xie Rongrong, Yang Jinkui

1. Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Diabetes Research and Care, Beijing Diabetes Institute, Beijing 100730, China

Received:2019-12-12 Online:2020-02-21 Published:2020-02-13
Supported by:
This study was supported by National Key P&D Program of China (2017YFC0909600);National Natural Science Foundation of China (81800688); Foundation of Beijing Tongren Hospital,Capital Medical University (TRYY-KYJJ-2016-013);Beijing Municipal Administration of Hospitals Incubating Program (PX2019006).

Abstract

Abstract: Objective To construct KCNH6 gene specific liver knockout mice and observe the changes of liver lipid metabolism. Methods KCNH6^flox/flox transgenic mice were constructed by Cas9 technique and hybridized with Alb-cre mouse which was specifically expressed Cre in liver cells. The genotype was identified by polymerase chain reaction (PCR). Weight and food intake in knockout mice and wildtype mice were detected. Triglycerides (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were assessed at 8 and 20 weeks, respectively. Results KCNH6 gene liver cell specific knockout mice were constructed, with the genotype of KCNH6^flox/flox/Cre^T. There were no significant changes in body weight and food intake of the mice in the control group. There was no significant change in lipid metabolism in male and female mice at 8 weeks. Cholesterol levels increased in male mice at 20 weeks. Conclusion The mouse model of KCNH6 gene liver cell specific knockout was constructed. Abnormal lipid metabolism was observed. The establishment of animal model provides a research platform to explore the role of KCNH6 gene in liver lipid metabolism.

Key words: KCNH6, conditional knockout, liver specific, Alb-cre, lipid metabolism

CLC Number:

R587

Lu Jing, Li Qi, Zhu Xiaorong, Xie Rongrong, Yang Jinkui. Construction and preliminary application of conditional KCNH6-knockout mice[J]. Journal of Capital Medical University, 2020, 41(1): 8-13.

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Construction and preliminary application of conditional KCNH6-knockout mice

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