Ang Ⅱ activates dendritic cell to produce immune function

doi:10.3969/j.issn.1006-7795.2017.05.016

Abstract

Abstract: Objective To investigate the immune effect of angiotensin Ⅱ(Ang Ⅱ) on immature dendritic cells (DC) compared with tumor necrosis factor-α (TNF-α)in vitro. Methods Prepared DC2.4 stimulated with different stimuli, such as 20 ng/mL TNF-α and 100 nmol Ang Ⅱ for indicated time. The effects of TNF-α and Ang Ⅱ on the proliferation, maturation, phagocytosis, cytokine secretion, migration, and communication with T cells of DCs are test by MTT, flow cytometry, ELISA, transwell assay and mixed lymphocyte culture respectively. Results First, the data showed that Ang Ⅱ stimulation significantly inhibited proliferation of DC, induced phenotypic maturation of DC, markedly increased the migration of DC, inhibited macrophage phagocytosis, promoted the secretion of interleukin-6 (IL-6) and interferon-γ(IFN-γ) and initiated the proliferation and activation of T-cell. Conclusion Compared with TNF-α, Ang Ⅱ positively modulated the function of DC to the same extent.

Key words: angiotensin Ⅱ (Ang Ⅱ), tumor necrosis factor-α, dendritic cell, immunity

CLC Number:

R392

Chen Chen, Meng Yan. Ang Ⅱ activates dendritic cell to produce immune function[J]. Journal of Capital Medical University, 2017, 38(5): 709-714.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.05.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I5/709

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