Dynamic changes of CD8+ stem memory T cells and their effects on disease progression in chronic HIV-1 infection

doi:10.3969/j.issn.1006-7795.2017.05.004

Abstract

Abstract: Objective To study the dynamics of CD8⁺ stem memory T cells (TSCM) and the impact of CD8⁺ TSCM cells on disease progression of human immunodeficiency virus-1 (HIV-1) infection. Methods Twenty-five cases with chronic HIV-1 infection receiving antiretroviral therapy (ART) with tenofovir plus efavirenz + lamivudine and 36 healthy controls were enrolled and observed. Peripheral blood mononuclear cells were stained with using CD3-APCCy7, CD4-FITC, CD8-PerCPCy5, CD45RA-PECy7, CCR7-APC, CD27-PE, CD95-Pacific Blue, CD38-PE, HLA DR-APC monoclonal antibodies, then CD8⁺ TSCM cell percentage were determined by flow cytometry.To compare the difference of CD8⁺ TSCM cell percentage between HIV-1 chronic infected persons and healthy subjects, and to analyze the relationship between CD8⁺ TSCM cell percentage and disease progression index (CD4⁺ T cell count, HIV-1 viral load and immune activation index).Results Chronic HIV-1 infection resulted in a decrease of the CD8⁺ TSCM cell proportion in HIV-1 patients. CD8⁺ TSCM cells positively correlated with CD4⁺ T cell counts and negatively correlated with plasma viral load and CD8⁺ T cell activation. Prolonged ART successfully recovered the CD8⁺ TSCM cells, and the dynamic change of CD8⁺ TSCM cells was in parallel with CD4⁺ T cell restoration and a decrease in the level of T cell immune activation. Conclusion In summary, this report identifies CD8⁺ TSCM as a correlate of protection from disease progression. HIV-1-specific CD8 ⁺ TSCM can presumably directly contribute to the design of T cell-based vaccines.

Key words: human immunodeficiency virus, stem memory T cells, disease progression

CLC Number:

R512

Song Bingbing, Lu Xiaofan, Weng Wenjia, Su Bin, Zhang Tong, Gao Yanqing. Dynamic changes of CD8⁺ stem memory T cells and their effects on disease progression in chronic HIV-1 infection[J]. Journal of Capital Medical University, 2017, 38(5): 650-653.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.05.004

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I5/650

References

[1] Farber D L, Yudanin N A, Restifo N P. Human memory T cells:generation, compartmentalization and homeostasis[J].Nat Rev Immunol,2014,14(1):24-35.
[2] Lugli E, Dominguez M H, Gattinoni L,et al. Superior T memory stem cell persistence supports long-lived T cell memory[J]. J Clin Invest,2013,123(2):594-599.
[3] Dudley M E, Wunderlich J R, Shelton T E, et al. Generation of tumor-infiltrating lymphocyte cultures for use in adoptive transfer therapy for melanoma patients[J]. J Immunother,2003,26:332-342.
[4] Stroncek D F, Berger C, Cheever M A, et al. New directions in cellular therapy of cancer:a summary of the summit on cellular therapy for cancer[J]. J Transl Med,2012,10:48.
[5] Zhou J, Shen X, Huang J, et al. Telomere length of transferred lymphocytes correlates with in vivo persistence and tumor regression in melanoma patients receiving cell transfer therapy[J]. J Immunol, 2005,175(10):7046-7052.
[6] Louis C U, Savoldo B, Dotti G, et al. Antitumor activity and long-term fate of chimeric antigen receptor-positive T cells in patients with neuro-blastoma[J]. Blood,2011,118(23):6050-6056.
[7] Gattinoni L, Lugli E, Ji Y, et al. A human memory T cell subset with stem cell-like properties[J]. Nat Med,2011,17(10):1290-1297.
[8] Lugli E, Gattinoni L, Roberto A,et al. Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells[J]. Nat Protoc,2013,8(1):33-42.
[9] Cartwright E K, McGary C S, Cervasi B,et al. Divergent CD4⁺ T memory stem cell dynamics in pathogenic and nonpathogenic simian immunodeficiency virus infections[J]. J Immunol,2014,192(10):4666-4673.
[10] Buzon M J, Sun H, Li C, et al. HIV-1 persistence in CD4⁺ T cells with stem cell-like properties[J]. Nat Med,2014,20(2):139-142.
[11] Jaafoura S, de Goer de Herve M G, Hernandez-Vargas E A, et al. Progressive contraction of the latent HIV reservoir around a core of less-differentiated CD4⁺ memory T Cells[J]. Nat Commun,2014,5:5407.
[12] Klatt N R, Bosinger S E, Peck M, et al. Limited HIV infection of central memory and stem cell memory CD4⁺ T cells is associated with lack of progression in viremic individuals[J].PLoS Pathog, 2014,10(8):e1004345.
[13] Walker B D, Chakrabarti S, Moss B, et al. HIV-specific cytotoxic T lymphocytes in seropositive individuals[J].Nature,1987,328(6128):345-348.
[14] Betts M R, Nason M C, West S M, et al. HIV non-progressors preferentially maintain highly functional HIV-specific CD8⁺ T cells[J]. Blood,2006,107(12):4781-4789.
[15] Giorgi J V, Hultin L E, McKeating J A, et al. Shorter survival in advanced human immunodeficiency virus type 1 in-fection is more closely associated with T lymphocyte activation than with plasma virus burden or virus chemokine coreceptor usage[J]. J Infect Dis,1999,179(4):859-870.

Dynamic changes of CD8⁺ stem memory T cells and their effects on disease progression in chronic HIV-1 infection

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Meng Huan, Wang Shuang, He Chaonan, Han Ying, Pan Meichen, Yin Shangqi, Zheng Mei, Jin Fangfang, Wang Yajie. The value of SAT detection ofhuman cytomegalovirus pp67 mRNA in the clinical diagnosis and treatment of human immunodeficiency virus patients with human cytomegalovirus infection [J]. Journal of Capital Medical University, 2021, 42(5): 739-746.
[2]	Wang Yanxue, Hu Hongying, Li Xingang, Lu Xingmeng, Gao Guiju, Liang Hongyuan, Xiao Jiang, Yang Di, Wang Fang, Han Xiaotao. A study of metabolic syndrome and cardiovascular disease risk status in highly active antiretroviral therapy-naive patients with human immunodeficiency virus infection [J]. Journal of Capital Medical University, 2020, 41(1): 40-44.
[3]	Wang Kaili, Chen Shizhang. Risk assessment and management of human immunodeficiency virus (HIV) patients with teeth extraction perioperative period [J]. Journal of Capital Medical University, 2019, 40(5): 754-757.
[4]	Zhan Yi, Shi Yuxin. Review for the HIV-associated neurocognitive disorders by magnetic resonance imaging [J]. Journal of Capital Medical University, 2016, 37(4): 481-485.
[5]	Tian Fei, Wang Yuanyuan, Ma Yunfang, Wang Jing, Kong Lingkun. Investigation of two intervention models to men who have sex with men in Dongcheng District, Beijing [J]. Journal of Capital Medical University, 2016, 37(2): 233-237.
[6]	Li Zhen, Wu Hao, Zhang Tong. Analysis of T cell activation in early HIV-infected individuals from MSM population [J]. Journal of Capital Medical University, 2014, 35(1): 92-95.
[7]	Xu Yuanlong, Yang Xiansan, Liu Kaiyan, Yi Yin, Li Qunhui, Huang Xiaojie, Guo Caiping, Wu Hao. Prevalence of and associated factors for HIV and HBV infections among men who have sex with men in Beijing, China [J]. Journal of Capital Medical University, 2014, 35(1): 96-100.
[8]	Tian Yaru, Jiao Yanmei, Zhang Tong, Wu Hao. Recent progress in the gene therapies against HIV-1 [J]. Journal of Capital Medical University, 2014, 35(1): 101-107.
[9]	DING Wei;ZHANG Yu-lin;QIAO Lu-xin;SONG Feng-li;XU Shu-ying;CHEN De-xi. Role of oxidative damage in HIV-related neurocognitive impairment [J]. Journal of Capital Medical University, 2012, 33(5): 602-609.
[10]	HAN Jie;ZHU Wei;LUN Wen-hui;LIAN Shi. Present status of research on pruritic papular eruption in patients with HIV infection [J]. Journal of Capital Medical University, 2011, 32(4): 476-478.
[11]	LI Juan;JIAO Yan-mei;GAO Yan-qing;KOU Bu-xin;ZHANG Tong;WU Hao. Optimization of Alu-LTR PCR approach to detect integrated HIV-1 DNA in variety of cell subsets at different stages after HAART [J]. Journal of Capital Medical University, 2011, 32(4): 449-452.
[12]	SHI Ying△;SUN Yu△;ZHANG Hong-hai;JI Yun-xia;QIAO Lu-xin;DING Wei;ZHANG Yu-lin;CHEN De-xi. Detection of plasma lactic acid in HIV-1 infected children treated withanti-viral therapy and analysis of relevant factors [J]. Journal of Capital Medical University, 2011, 32(3): 337-340.
[13]	JIAO Yan-mei;FU Jun-liang;ZHANG Zheng;JI Yun-xia;WANG Rui;ZHANG Tong;CHEN De-xi;WU Hao. Treg Cells Depletion Can Lead to HIVspecific CD8+Cells Apoptosis in HIV Patients [J]. Journal of Capital Medical University, 2011, 32(2): 186-189.
[14]	LIANG Hong-xia;YU Zu-jiang;ZHANG Qian;LI Zhi-qin;PAN Yan-feng;LI Juan;JIANG He-qing;LI Jian-sheng;HE Yun. Influences of HCV Infection on Incidence of Occult HBV Infection in Patients Infected with HIV-1 [J]. Journal of Capital Medical University, 2011, 32(2): 190-193.
[15]	ZHANG Hong-wei;WU Hao. Structural Characteristics and Biological Effects of HIV Negative Factor [J]. Journal of Capital Medical University, 2011, 32(2): 199-202.