Effects of tetrahydroxystilbene glucoside on amyloid precursor protein expression in nerve cells and its mechanism related to CREB regulation

doi:10.3969/j.issn.1006-7795.2017.06.012

Journal of Capital Medical University ›› 2017, Vol. 38 ›› Issue (6): 835-840.doi: 10.3969/j.issn.1006-7795.2017.06.012

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Effects of tetrahydroxystilbene glucoside on amyloid precursor protein expression in nerve cells and its mechanism related to CREB regulation

Yin Xiaomin^1,2, Xu Ting², Dai Wufei², Qian Jiayi², Chen Chen¹, Zhang Lan¹, Li Lin¹

1. Department of Pharmacology, Xuanwu Hospital, Capital Medical University, Beijing Engineering Research Center for Nerve System Drugs, Beijing 100053, China;
2. Department of Biochemistry, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China

Received:2017-10-01 Online:2017-11-21 Published:2017-12-16
Supported by:
This study was supported by National Natural Science Foundation of China (81273498, 81473373, 81503077), National Science and Technology Major Project for New Drug Research and Development of China (2015ZX09101-016), Jiangsu Students' Innovation and Entrepreneurship Training Program (201610304095x).

Abstract

Abstract: Objective To explore the effects of tetrahydroxystilbene glucoside (TSG) on the expression of amyloid precursor protein (APP) and its mechanism in cultured nerve cells.Methods The expression of APP and cAMP responsive element-binding protein (CREB) was detected by Western blotting assay. The CRE cis-elements of APP promoter were analyzed by Matinspector software. The plasmids expressing serial deletion mutants of APP promoter were constructed. The luciferase activity in cells was measured by luciferase double reporter gene system. Results Activation of CREB by Forskolin down-regulated APP expression in SH-SY5Y cells. The luciferase expression plasmids of APP promoter containing CRE elements were successfully constructed. TSG treatment decreased APP level in SH-SY5Y cells, and reduced luciferase activity of APP reporter gene containing CRE1 and CRE2 cis-elements in APP promoter plasmids-transfected cells.Conclusion TSG down-regulated APP expression in nerve cells, and its mechanism may be related to affecting the binding of CREB with CRE cis-elements.

Key words: tetrahydroxystilbene glucoside, amyloid precursor protein, cAMP responsive element-binding protein, Alzheimer's disease

CLC Number:

R749.1

Yin Xiaomin, Xu Ting, Dai Wufei, Qian Jiayi, Chen Chen, Zhang Lan, Li Lin. Effects of tetrahydroxystilbene glucoside on amyloid precursor protein expression in nerve cells and its mechanism related to CREB regulation[J]. Journal of Capital Medical University, 2017, 38(6): 835-840.

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Effects of tetrahydroxystilbene glucoside on amyloid precursor protein expression in nerve cells and its mechanism related to CREB regulation

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