Effects of Xiaoshuan enteric-coated capsule on neuronal damage by eliminating β-amyloid protein in thalamus of MCAO rats

doi:10.3969/j.issn.1006-7795.2015.03.016

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (3): 420-425.doi: 10.3969/j.issn.1006-7795.2015.03.016

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Effects of Xiaoshuan enteric-coated capsule on neuronal damage by eliminating β-amyloid protein in thalamus of MCAO rats

Zhang Ning^1,2,3, Wu Xi⁴, Wang Shuo⁴, Wang Lei⁵, Zhang Qiuxia⁵, Zhao Hui⁵, Wang Yali⁵

1. Neuropsychiatry&Behavioral Neurology and Clinical Psychology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;
2. China National Clinical Research Center for Neurological Diseases, Beijing 100050, China;
3. Center of Stroke, Beijing Institute for Brain Disorders, Beijing 100050, China;
4. Neurology Department Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;
5. Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China

Received:2014-11-20 Online:2015-06-21 Published:2015-06-15
Supported by:
This work was supported by the National Natural Science Foundation of China (81370482 and 31400991),Natural Science Foundation of Beijing(7132017), the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality (IDHT20140514).

Abstract

Abstract:

Objective To investigate the effects of Xiaoshuan enteric-coated capsule on neuronal damage and the expression of cystein-aspartate protease-3 (Caspase-3), amyloid precursor protein (APP) and β- amyloid protein (Aβ) in thalamus of middle cerebral artery occlusion(MCAO)rats. Methods The animal model of middle cerebral artery occlusion(MCAO)were adopted. Xiaoshuan enteric-coated capsule(420、140、47 mg·kg^-1) was given orally to rats once each day for 15 days after ischemia. The expression of Caspase-3 was detected by using immunofluorescence and APP, Aβ42 were detected by using immunohistochemistry at 15d after ischemia. Results Compared with control rats, the number of neurons was reduced and the number of Caspase-3 was added, the expression of APP and Aβ42 was increased in MCAO rats(P <0.01); The number of neurons was added and the number of Caspase-3 was reduced, the expression of APP and Aβ42 was decreased in Xiaoshuan enteric-coated capsule rats to compared with the model group(P <0.05). Conclusion Xiaoshuan enteric-coated capsule increased the number of neurons, reduced the number of Caspase-3 and decreased the expression of APP and Aβ42. As a result, Xiaoshuan enteric-coated capsule can protect the thalamus of ischemia.

Key words: Xiaoshuan enteric-coated capsule, cerebral ischemia, cystein-aspartate protease-3, amyloid precursor protein, β-amyloid protein

CLC Number:

R741

Zhang Ning, Wu Xi, Wang Shuo, Wang Lei, Zhang Qiuxia, Zhao Hui, Wang Yali. Effects of Xiaoshuan enteric-coated capsule on neuronal damage by eliminating β-amyloid protein in thalamus of MCAO rats[J]. Journal of Capital Medical University, 2015, 36(3): 420-425.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.03.016

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Effects of Xiaoshuan enteric-coated capsule on neuronal damage by eliminating β-amyloid protein in thalamus of MCAO rats

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