The localization of dopamine receptors on the nitrergic neurons in gastric myenteric plexus of rats

doi:10.3969/j.issn.1006-7795.2015.03.017

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (3): 426-431.doi: 10.3969/j.issn.1006-7795.2015.03.017

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The localization of dopamine receptors on the nitrergic neurons in gastric myenteric plexus of rats

Fan Ruifang, Zheng Lifei, Feng Xiaoyan, Zhang Xiaoli, Zhang Yue, Zhu Jinxia

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2015-02-26 Online:2015-06-21 Published:2015-06-15
Supported by:
This work was supported by the National Natural Science Foundation of China (81370482 and 31400991), Natural Science Foundation of Beijing (7132017), the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality (IDHT20140514).

Abstract

Abstract:

Objective To investigate the distribution of dopamine receptors in neural nitric oxide synthase (nNOS)immunoreactive neurons in myenteric plexus of gastric corpus. Methods Immunofluorescence for frozen sections of gastric corpus and whole mount stretched preparation of myenteric plexus were used to investigate the colocolization of dopamine receptors and nNOS in myenteric neurons. Results The immunoreactivities of five subtypes' dopamine receptors and nNOS were largely distributed in the enteric neurons of gastric myenteric plexus. Furthermore, the Immunofluorescence of dopamine receptor D₁, D₂, D₃ and D₅, but not D₄ were observed in the nNOS-immunoreactive neurons. Conclusion Dopamine receptor D₁, D₂, D₃ and D₅ were distributed in the gastric nitrergic neurons of gastric corpus, which might be involved in the NO synthesis, and then regulation of gastric motility.

Key words: dopamine receptors, gastric myenteric plexus, nitrergic neuron

CLC Number:

R333

Fan Ruifang, Zheng Lifei, Feng Xiaoyan, Zhang Xiaoli, Zhang Yue, Zhu Jinxia. The localization of dopamine receptors on the nitrergic neurons in gastric myenteric plexus of rats[J]. Journal of Capital Medical University, 2015, 36(3): 426-431.

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The localization of dopamine receptors on the nitrergic neurons in gastric myenteric plexus of rats

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