Expression and cellular distribution of dopamine receptors in the rat colonic submucosal plexus

doi:10.3969/j.issn.1006-7795.2017.03.017

Abstract

Abstract: Objective Dopamine (DA) plays an important role in the regulation of intestinal motility, secretion and mucosal barrier by binding to its receptors. It has been shown that submucosal neurons are involved in the regulation effect of DA on the mucosa function. However the expressions of DA receptors in submucosal neurons are not clear. The aim of the present study is to investigate the distribution of DA receptors in vasoactive intestinal peptide (VIP) neurons and cholinergic neurons in the submucosa. Methods The mRNA and protein expression of DA receptors was measured by RT-PCR and Western blotting. Immunofluorescent double labeling of DA receptors with submucosal VIP neurons and cholinergic neurons were observed under confocal laser scanning microscope. Results Both the mRNA and protein of DA receptors D₁, D₂ and D₅ were expressed in the colonic submucosal layer. In the submucosal plexus, D₁ positive VIP-positive neurons accounting for 58.94%±2.245%, D₂ for 52.03%±9.384%, and D₅ for 86.21%±2.902%. The number of D₅ positive neurons was significantly higher than that of D₁ and D₂. In the submucosal plexus, D₁ positive cholinergic neurons accounted for 87.75%±7.307%, D₂ for 88.50%±8.761%, and D₅ for 89.25%±10.75%, and the distribution of the three types of DA receptors in the cholinergic neurons had no significant difference. Conclusion DA receptors D₁, D₂ and D₅ are expressed in the submucosal layer of the rat colon. And they all are distributed in the submucosal VIP and cholinergic neurons, and D₅ expression was higher than D₁ and D₂ in VIP-positive neurons. Our study provides a morphological basis for the regulation of DA on the colonic mucosal function.

Key words: dopamine, dopamine receptors, submucosal plexus, vasoactive intestinal peptide, acetylcholine

CLC Number:

R392

Zhang Yue, Li Yun, Zhu Jinxia, Zhao Wenming. Expression and cellular distribution of dopamine receptors in the rat colonic submucosal plexus[J]. Journal of Capital Medical University, 2017, 38(3): 411-416.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.03.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I3/411

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