Protective effect of tea polyphenols on dopaminergic neuron in Parkinsonism disease monkeys

doi:10.3969/j.issn.1006-7795.2015.05.005

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (5): 689-693.doi: 10.3969/j.issn.1006-7795.2015.05.005

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Protective effect of tea polyphenols on dopaminergic neuron in Parkinsonism disease monkeys

Chen Hongwei¹, Yu Lan^2,3, Chen Min^2,3, Li Xuran^2,4, Li Xin^2,4, Yang Weiwei^2,4, Wang Peng^2,4, Yu Shun^2,4,5

1. Chaoyang District Shibalidian Community Health Service Centers, Beijing 100122, China;
2. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
3. Department of Human Anatomy, Guilin Medical University, Guilin 541004, China;
4. Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China;
5. Center for Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100053, China;

Received:2015-09-14 Online:2015-10-21 Published:2015-10-20
Contact: 于顺 E-mail:yushun103@163.com
Supported by:
This study was supported by Major State Basic Research Program (973 Program) of China (2011CB504101), National Natural Science Foundation of China (81071014, 81371200, 81401042), National Science and Technology Support Program (2012BAI10B03), Natural Science Foundation of Beijing (7122035), Capital Health Research and Development Special Fund (2011-4001-01).

Abstract

Abstract: Objective To investigate the protecting efficacy of tea polyphenols on dopaminergic neurons in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced Parkinson's disease monkeys and its mechanism. Methods Healthy female cynomolgus monkeys 10 to 12 years old were randomly divided into four groups: normal control group (n= 4), TP group (n=4), MPTP group (n=4), and MPTP + TP group (n=4). Control group were not given any treatment; The TP group monkeys were treated with TP by gastrogavage once daily for 80 consecutive days. In MPTP and MPTP+TP groups, MPTP was injected intravenously as MPTP-HCl to induce PD model. The MPTP+TP groups were treated with TP by gastrogavage once daily for 80 consecutive days after making PD model. Motor deficits were quantified twice a week by a previously validated Parkinsonian monkey clinical rating scale. All animals were euthanized and the brains tissues were rapidly separated. To measure the levels of dopamine and its metabolites in the striatum were measured using HPLC method. Dopaminergic neurons were identified by immunohistochemistry and stereology. Results Tea polyphenols alleviated MPTP-induced injury of nigral dopaminergic neurons and improved motor impairments in cynomolgus monkeys (P<0.05). Conclusion This study showed first in vivo evidence that TP could alleviate dopaminergic neuronal injury and motor impairments in nonhuman primates.

Key words: tea polyphenols, motor impairments, dopaminergic neuronal, Parkinson's disease

CLC Number:

R338

Chen Hongwei, Yu Lan, Chen Min, Li Xuran, Li Xin, Yang Weiwei, Wang Peng, Yu Shun. Protective effect of tea polyphenols on dopaminergic neuron in Parkinsonism disease monkeys[J]. Journal of Capital Medical University, 2015, 36(5): 689-693.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.05.005

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Protective effect of tea polyphenols on dopaminergic neuron in Parkinsonism disease monkeys

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