Inhibitive effects on MPTP induced alpha-synuclein aggregation by tea polyphenols and EGCG

doi:10.3969/j.issn.1006-7795.2015.05.003

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (5): 680-683.doi: 10.3969/j.issn.1006-7795.2015.05.003

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Inhibitive effects on MPTP induced alpha-synuclein aggregation by tea polyphenols and EGCG

Yu Lan^1,2, Chen Min^1,2, Li Xuran^1,3, Li Xin^1,3, Yang Weiwei^1,3, Yu Shun^1,3,4

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Department of Human Anatomy, Guilin Medical University, Guilin 541004, Guangxi Zhuang Autonomous Region, China;
3. Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China;
4. Center for Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100053, China

Received:2015-09-14 Online:2015-10-21 Published:2015-10-20
Contact: 于顺 E-mail:yushun103@163.com
Supported by:
This study was supported by Major State Basic Research Development Program of China (973 Program)(2011CB504101), National Natural Science Foundation of China (81071014, 81371200, 81401042), National Science and Technology Support Program (2012BAI10B03), Natural Science Foundation of Beijing (7122035),The Capital Health Research and Development Special Fund (2011-4001-01).

Abstract

Abstract: Objective To investigate the inhibitive effects of tea polyphenols (TP) and their extract epigallocatechin-3-gallate (EGCG) on -synuclein aggregation in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-intoxicated Parkinson's disease. MethodsHealthy female 10 to 12 years old cynomolgus monkeys were randomly divided into normal control group (n=4), TP group (n=4), MPTP group (n=4), and MPTP+TP group (n=4). Control group monkeys were not given any treatment; The monkeys in TP group were treated with TP by gastrogavage once daily for 80 consecutive days. In MPTP and MPTP+TP groups, MPTP was injected intravenously as MPTP-HCl to induce PD model. The MPTP+TP groups were treated with TP by gastrogavage once daily for 80 consecutive days after establishing PD model. All animals were euthanized and the brains tissues were rapidly isolated. The level of α-syn oligomers were measured by the ELISA method. In vitro, MES23.5 dopaminergic neurons treated with α-syn monomers or oligomers, followed by treatment with vehicle MPP+ and (or) EGCG. Intracellular levels of α-syn oligomers were measured by ELISA. Cell viability was estimated using the MTT formazan colorimetric assay. Results MPTP-intoxication could increase the levels of α-syn oligomers and TP administration could reduce α-syn oligomers in the brain of MPTP-treated monkeys. In vitro study demonstrated while addition of MPP+ significantly elevated intracellular levels of α-syn in untreated control group and groups treated with α-syn monomers and α-syn oligomers. The capacity of the extract EGCG to reduce -syn oligomerization was further examined and alleviated the MPP+induced cell injury in cultured cells. Conclusion The tea polyphenols and its extract EGCG could alleviate -syn aggregation and dopaminergic neuronal injury caused by MPTP-intoxication.

Key words: tea polyphenols, α-synuclein, dopaminergic neurons cell

CLC Number:

R338

Yu Lan, Chen Min, Li Xuran, Li Xin, Yang Weiwei, Yu Shun. Inhibitive effects on MPTP induced alpha-synuclein aggregation by tea polyphenols and EGCG[J]. Journal of Capital Medical University, 2015, 36(5): 680-683.

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Inhibitive effects on MPTP induced alpha-synuclein aggregation by tea polyphenols and EGCG

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