Neuronal hemoglobin in mitochondria is reduced by forming a complex with α-synuclein

doi:10.3969/j.issn.1006-7795.2016.05.013

Abstract

Abstract: Objective In this study, we observed whether α-synuclein (α-syn) and neuronal hemoglobin (nHb) formed a complex in different subcellular pool of brain regions in aging cynomolgus monkeys. Methods The levels of nHb-α-syn complex were detected in different subcellular pools of different regions in aging monkey brains using co-immunoprecipitation (Co-IP) and enzyme linked immunosorbent assay (ELISA). The expressions of free α-syn and nHb were detected in different subcellular pools of aging monkey brains by Western blotting. Results In the striatum, cytoplasmic nHb-α-syn complex increased with age, while mitochondrial nHb-α-syn complex decreased with age and was accompanied by a reduction in mitochondrial free nHb (P<0.05). In contrast, no changes in nHb-α-syn complex formation or free nHb levels were detected in the cerebellum in young, middle age and aged monkey brains. Conclusion The above results suggest that α-syn forms a complex with nHb in selected regions of the aging brain.

Key words: α-synuclein (α-syn), hemoglobin, aging, mitochondria

CLC Number:

R338

Yang Weiwei, Li Xuran, Li Xin, Li Xuying, Yu Shun. Neuronal hemoglobin in mitochondria is reduced by forming a complex with α-synuclein[J]. Journal of Capital Medical University, 2016, 37(5): 621-625.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.05.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I5/621

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