Role of α-synuclein in microglia inflammation and phagocytosis

doi:10.3969/j.issn.1006-7795.2018.05.013

Abstract

Abstract: Objective Our study observed the effect of α-synuclein (α-syn) on inflammation and phagocytosis of microglia which are the resident immune cells in central nervous system. Methods Primary microglia cultured in vitro were treated by different concentrations of α-syn or lipopolysaccharides (LPS). Then the changes of phagocytosis of microglia were observed by fluorescent microspheres and fluorescent staining with clusters of differentiation 68 (CD68) proteins.The expression of inflammatory factors,tumor necrosis factor-α (TNF-α) and interleukin-1 beta (IL-1β), were detected by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) to observe the α-syn induced inflammatory response. Results Twenty-four hours after α-syn stimulation, the number of microspheres that ingested in cells and CD68 expression increased significantly, similar to that with LPS treatment. However, both the changes of transcription level of inflammatory factors, TNF-α and IL-1β, and the release of TNF-α stimulated by α-syn were much lower than those by LPS. Conclusion α-syn can significantly enhance the phagocytosis of microglia, but has little effect on inflammatory response.

Key words: α-synuclein, phagocytosis, inflammation

CLC Number:

R741.02

Gong Xiaoli, Liu Mengru, Wang Le, Liu Yang, Zhang Ting, Sun Ying, Wang Xiaomin. Role of α-synuclein in microglia inflammation and phagocytosis[J]. Journal of Capital Medical University, 2018, 39(5): 693-698.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.05.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I5/693

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