Effects of icariin on the behavioral changes, demyelination and neuroinflammation in mice with cuprizone-induced schizophrenia

doi:10.3969/j.issn.1006-7795.2021.05.011

Abstract

Abstract: Objective To investigate the effects of icariin (ICA) on the behavioral and pathological changes in the mice with cuprizone induced schizophrenia. Methods The model was established by feeding 0.2% cuprizone-containing fodder for 5 weeks in C57BL/6 mice. The behavioral changes were detected by open-field test and Y-maze test. The oil red O staining was applied to observe the demyelination, and the immunohistochemical method was used to detect the expression of myelin basic protein (MBP) and the number of microglia and astrocytes. Results Compared with the control group, the cuprizone model group showed a lower spontaneous alternation rate (decreased spatial working memory) in the Y-maze test and a longer total course of spontaneous activity (high activity positive symptom) in the open-field test. Moreover, the demyelination, decreased expression of MBP, and increased number of microglia and astrocyte were observed in the corpus callosum of model mice. Intragastric administration of ICA for 5 weeks significantly improved the spatial working memory impairment and the positive symptoms of high activity, reduced the demyelination, increased the expression of MBP, and inhibited the activation of microglia and astrocytes in the corpus callosum of cuprizone model mice. Conclusion ICA could improve the positive symptom and cognitive impairment in the mice with cuprizone-induced schizophrenia, and the mechanisms might be related to the inhibitory effects of ICA on the demyelination and neuroinflammation. These results suggested that ICA may have the potential to treat schizophrenia.

Key words: icariin, schizophrenia, cuprizone, demyelination, neuroinflammation

CLC Number:

R745.1

Chen Xi, Ma Denglei, Li Lin. Effects of icariin on the behavioral changes, demyelination and neuroinflammation in mice with cuprizone-induced schizophrenia[J]. Journal of Capital Medical University, 2021, 42(5): 761-767.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.05.011

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I5/761

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