Effects of Yishenpingganfang, an empirical prescription on social interaction behavior in mice exposed to cuprizone and its possible action target

doi:10.3969/j.issn.1006-7795.2019.02.011

Abstract

Abstract: Objective To explore the effect and potential target of Yishenpingganfang (YSPGF) in schizophrenia treatment. Methods Totally 30 Young adult male C57BL/6 mice (6 weeks old) were randomly divided into three groups:normal control, cuprizone (CPZ) model, and YSPGF group. This study lasts for 6 weeks. Then, social interaction tests and brain tissue collections were carried out 24h later after 2-week period of drug admission. Social interaction was used to evaluate schizophrenia-like symptoms in mice exposed to cuprizone and partly treated by YSPGF. The mature oligodendrocyte/myelin deficiency and immature oligodendrocyte changes were observed with histological staining. Results The body weight in the model group were obviously decreased in week 3 compared with the control group (P<0.05). Social interaction included two trials:In trial 1, control mice spent more time interacting with the stranger mouse than with the empty cage and familiar one (P<0.001) as well as YSPGF group (P<0.01).In trial 2, control mice preferred to be near the new stranger mouse than the familiar one (P<0.001). In contrast, CPZ mice showed no preference between the empty cage and the stranger mouse in both trials 1 and 2.The oligodendrocyte/myelin deficiency existed in callosum through using mature oligodendrocyte maker myelin basic protein (MBP). Furthermore, we used immature oligodendrocyte maker platelet-derived growth factor receptor-alpha (PDGFR-α) and found that in model group mice these immature oligodendrocyte increased after the demyelination stage. Conclusion YSPGF could improve CPZ-induced psychosis-like behaviors and reduce oligodendrocyte/myelin deficiency.

Key words: schizophrenia, Yishenpingganfang, oligodendrocyte, cuprizone, social interaction

CLC Number:

Wu Yan, Sun Zuoli, Ma Chao, Zhu Hong, Yin Dongqing, Jia Hongxiao. Effects of Yishenpingganfang, an empirical prescription on social interaction behavior in mice exposed to cuprizone and its possible action target[J]. Journal of Capital Medical University, 2019, 40(2): 209-213.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.02.011

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I2/209

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