Analysis of effect of Shi-Zhen-An-Shen-Tang on intestinal microflora diversity of mice exposed to cuprizone based on 16S rRNA technique

doi:10.3969/j.issn.1006-7795.2020.01.013

Abstract

Abstract: Objective To explore the effect of Shi-Zhen-An-Shen-Tang(SZAST) on intestinal microflora diversity of demyelinated model mice, and further to reveal its therapeutic characteristics and mechanisms based on the 16S rRNA technique. Methods Thirty Young adult male C57BL/6 mice (5 weeks old) were randomly divided into six groups, each group of 5 mice:normal control, model, and low dosage of SZAST, medium dosage of SZAST, high dosage of SZAST and quetiapine(QTP)-treated group. The control group were given mixed cuprizone (CPZ, a copper chelator, 0.2%, w/w) rodent chow for six successive weeks to induce demyelination. During the last two weeks, mice were given an oral gavage of saline, or SZAST of three different doses (a low dose of 5.5 g·kg^-1·d^-1, a medium dose of 11.0 g·kg^-1·d^-1, or a high dose of 16.5 g·kg^-1·d^-1), or QTP, respectively. Then, the mice were anesthetized and their cecum contents removed. Illumina MiSeq was used as the sequencing platform to analyze and evaluate the number of operational taxonomic units (OTUs), richness and diversity indexes, diversity of alpha and beta, differential phylum and genus of intestinal flora in V3-V4 zone of 16S rRNA. Results SZAST could regulate the number of OTUs, richness and diversity indexes of intestinal flora, imbalance of alpha and beta diversity of demyelinated model mice.Compared to the control group, Lachnospiraceae family were decreased in the model group (p<0.05), and SZAST could increase the diversity of Lachnospiraceae family. Compared to the control group, Beta-proteobacteria, Burkholderiales, Alcaligenaceae, and Bacteroidales were increased in the model group (p<0.05), but SZAST could decrease these intestinal flora. Conclusion SZAST had a therapeutic effect on the abnormal flora diversity of demyelinated model mice. It revealed the relevant pathological mechanism of mental schizophrenia caused by white matter damage through 16S rRNA technology.

Key words: schizophrenia, Shi-Zhen-An-Shen-Tang, cuprizone, gut microbiota, high-throughput sequencing, 16S rRNA technique, operational taxonomic units(OTUs)

CLC Number:

Ma Chao, He Yi, Sun Zuoli, Liu Xinyao, Feng Zhengtian, Chen Pei, Ning Yanzhe, Zhu Hong, Yin Dongqing, Jia Hongxiao. Analysis of effect of Shi-Zhen-An-Shen-Tang on intestinal microflora diversity of mice exposed to cuprizone based on 16S rRNA technique[J]. Journal of Capital Medical University, 2020, 41(1): 64-70.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.01.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I1/64

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