Cannabinoid receptor 1 mediated the migration of human neutrophil-like cell line dHL60

doi:10.3969/j.issn.1006-7795.2017.03.018

Journal of Capital Medical University ›› 2017, Vol. 38 ›› Issue (3): 417-422.doi: 10.3969/j.issn.1006-7795.2017.03.018

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Cannabinoid receptor 1 mediated the migration of human neutrophil-like cell line dHL60

Fan Xiaoting, Tian Lei, Yang Lin, Li Liying

Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing 100069, China

Received:2016-08-05 Online:2017-05-21 Published:2017-06-14
Supported by:
This study was supported by National Natural and Science Foundation of China (81430013), Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality (IDHT20150502).

Abstract

Abstract: Objective To investigate the effect of cannabinoid receptors (CBs) on migration of human neutrophil-like cell line dHL60. Methods HL60 cell was cultivated in vitro and stimulated by dimethylsulphoxide (DMSO) to differentiate into neutrophil-like cells (dHL60), RT-PCR was used to detect the CD11b mRNA expression as a differentiation marker. Western blotting and agarose gel electrophoresis were used to detect the expression of CB1 and CB2. Boyden chamber was used for cell migration assay. Filamentous actin (F-actin) stained with phalloidin was examined by high content analysis. Results In this study, we present evidence that dHL60 significantly expressed CB1 and CB2; the treatment of ACEA (agonist of CB1) caused an increase in dHL60 migration and F-actin remodeling, the pharmacological inhibition of CB1 markedly attenuated ACEA-induced migration; the treatment of JWH133 (agonist of CB2) has no effect on migration and F-actin remodeling. Conclusion CB1 mediated the migration of human neutrophil-like cell line dHL60.

Key words: cannabinoid receptors(CBs), human neutrophil-like cell, cell migration

CLC Number:

Fan Xiaoting, Tian Lei, Yang Lin, Li Liying. Cannabinoid receptor 1 mediated the migration of human neutrophil-like cell line dHL60[J]. Journal of Capital Medical University, 2017, 38(3): 417-422.

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Cannabinoid receptor 1 mediated the migration of human neutrophil-like cell line dHL60

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