The expression of glial fibrillary acidic protein in gastric corpus of 6-OHDA rats

doi:10.3969/j.issn.1006-7795.2016.05.011

Journal of Capital Medical University ›› 2016, Vol. 37 ›› Issue (5): 611-615.doi: 10.3969/j.issn.1006-7795.2016.05.011

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The expression of glial fibrillary acidic protein in gastric corpus of 6-OHDA rats

Ren Qinzhan, Zheng Lifei, Liu Wei, Zhu Jinxia

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2015-12-18 Online:2016-10-21 Published:2016-10-19
Supported by:
This study was supported by National Natural Science Foundation of China (81170346, 81370482, 31400991), Natural Science Foundation of Beijing (7121003, 7132017), Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality (IDHT20140514) and Basic-Clinical Scientific Research Cooperation Project of Capital Medical University (14JL14).

Abstract

Abstract: Objective To study the glial fibrillary acidic protein (GFAP) expression in gastric corpus of 6-hydroxydopamine (6-OHDA) treated rats and explore the underlying mechanism of enteric glial cells in gastroparesis of Parkinson's disease (PD). Methods 6-OHDA PD rat model was made by microinjecting 6-OHDA into bilateral substantia nigra. Immunofluorescence was used to observe the distribution of tyrosine hydroxylase (TH) immunoreactive (IR) neurons in the substantia nigra and the expression of GFAP and neurofilament (NF) in gastric corpus of 6-OHDA rats. Western blotting was applied to detect the expression of GFAP and NF in gastric corpus of 6-OHDA rats. Results In rats treated with 6-OHDA, the number of TH-IR neurons in the substantia nigra was significantly reduced; Immunofluorescence results showed that both GFAP and NF expression were distinctly decreased in gastric corpus of 6-OHDA rats; Compared with the control rats, the protein level of GFAP was significantly decreased in gastric corpus of 6-OHDA rats by Western blotting. Conclusion The results suggest that decrease of NF and GFAP in gastric corpus may play a role in the course of gastroparesis of 6-OHDA rats, which may provide a new idea for prevention of gastroparesis in PD.

Key words: 6-hydroxydopamine rats, glial fibrillary acidic protein, neurofilament

CLC Number:

R363

Ren Qinzhan, Zheng Lifei, Liu Wei, Zhu Jinxia. The expression of glial fibrillary acidic protein in gastric corpus of 6-OHDA rats[J]. Journal of Capital Medical University, 2016, 37(5): 611-615.

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The expression of glial fibrillary acidic protein in gastric corpus of 6-OHDA rats

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