The effect of lncRNA FAM95B1 on the pathogenesis of intrauterine adhesion by regulating insulin-like growth factor 2

doi:10.3969/j.issn.1006-7795.2023.05.017

Abstract

Abstract: Objective To investigate the effect of long non-coding RNA (lncRNA) family with sequence similarity 95 member B1(FAM95B1) and insulin-like growth factor 2 (IGF2) on the pathogenesis of intrauterine adhesion. Methods Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine the mRNA expression levels of lncRNA FAM95B1, Western blotting was used to determine the protein expression levels of IGF2, α-smooth muscle actin (α-SMA) and collagen type I alpha 1 chain protein (COL1A1) in samples of intrauterine adhesion (IUA) tissue. RT-qPCR was used to determine the mRNA expression levels of lncRNA FAM95B1, IGF2, fibrotic markers (α-SMA, COL1A1, Snail ) and epithelial marker E-cadherin in endometrial stromal cells (ESCs) that had been treated with transforming growth factor-β1 (TGF-β1). Then, lncRNA FAM95B1 and IGF2 genes were down-regulated respectively to verify the regulatory relationship between lncRNA FAM95B1 and IGF2; Co-immunoprecipitation assay was performed to measure the binding relationship between lncRNA FAM95B1 and IGF2. Next, ESCs were transfected with si-FAM95B1 and then were treated with TGF-β1 to investigate the effect of lncRNA FAM95B1 and IGF2 on the transdifferentiation of ESCs into myofibroblasts. Results The expression levels of lncRNA FAM95B1, IGF2, α-SMA and COL1A1 was upregulated in IUA tissues. The mRNA expression levels of lncRNA FAM95B1, IGF2, α-SMA, COL1A1 and Snail were upregulated and the mRNA level of E-cadherin was downregulated in ESCs that had been treated with TGF-β1. IGF2 was positively regulated by lncRNA FAM95B1. LncRNA FAM95B1 and IGF2 were directly bound in TGF-β1-treated ESCs. During the epithelial-to-mesenchymal transition（EMT） of ESCs transdifferentiating into myofibroblasts, si-FAM95B1 exerted antifibrotic effect by down-regulating IGF2. Conclusions si-FAM95B1 inhibits the pathogenesis of IUA by down-regulating IGF2. LncRNA FAM95B1/IGF2 axis may provide novel molecular target for the prevention and treatment of IUA.

Key words: intrauterine adhesions, LncRNA FAM95B1, insulin-like growth factor 2, epithelial-to-mesenchymal transition

CLC Number:

R711.74

Wu Jianhong, Tian Yucui, Jiang Ziwen, Tang Shiqian, Liu Zhaohui, Dai Yinmei. The effect of lncRNA FAM95B1 on the pathogenesis of intrauterine adhesion by regulating insulin-like growth factor 2[J]. Journal of Capital Medical University, 2023, 44(5): 821-827.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2023.05.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2023/V44/I5/821

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