关键词: 活体亲属肾移植, HLA 分型, 群体反应性抗体

Abstract: Objective To investigate correlation of posttransplant renal function with HLA typing and panel reactive antibody(PRA) in related living donor renal transplantation recipients. Methods Data from 336 patients who received living renal transplantation in our hospital, were analyzed. PRA, serum creatinine and urea nitrogen level were detected 1-4 years after transplantation. The donations were classified 5 groups: 1 Parents donated to sons and daughters; 2 Sons and daughters donated to parents; 3 Donation between brothers and sisters; 4 others; 5 Donation between husbands and wives. HLA-PCR-SSP kits were provided by One Lanmbda Company. PRA in recipients was detected by enzyme-linked immune absorbent assay. Results In group 1 118 recipients shared half-match HLA-A, B, DR, DQ with donors, 22 patients had more than half match; 36 recipients' renal function decreased and 8 patients PRA antibody positive post-transplantation. In group 2 12 recipients shared half-match HLA-A, B, DR, DQ with donors, 7 recipients more than half-match; 1 recipient had renal function decrease and underwent transplantation for the second time. In group 3, of 107 recipients, 18 recipients shared the same HLA-A, B, DR, DQ typing with donors, 73 recipients had more than half-match or half-match, others lower half-match or mismatch; 13 recipients renal function decreased and 3 recipients were PRA antibody positive post-transplantation. In group 4, of 92 recipients, 24 shared half-match with donors, 9 recipients were mismatched, although HLA typing matched for more than 4 antigens, 8 recipients had non-half-match, 51 recipients had 3 antigens or less match; 11 recipients had renal function decrease and 6 recipients PRA antibody positive in post-transplantation. In group 5 all the 7 recipients had HLA typing rate less 3 antigens; 2 recipients renal function decreased and PRA antibody positive post-transplantation. Conclusion The rate of matched HLA typing between the immediate family members was higher than that in other relative donation, however, the rate of completely matched HLA typing was lower between brothers and sisters. There was no correlation between HLA typing and renal allograft function in the near future, but HLA typing was relevant to donor' age. Well-matched HLA typing was correlated with low level PRA post renal transplantation.

Key words: living renal transplantation, human leukocyte antigen typing, panel reactive antibody