探讨γ-谷氨酰基转移酶冰冻混合人血清候选标准物质的制备和稳定性

doi:10.3969/j.issn.1006-7795.2012.02.007

首都医科大学学报 ›› 2012, Vol. 33 ›› Issue (2): 165-168.doi: 10.3969/j.issn.1006-7795.2012.02.007

探讨γ-谷氨酰基转移酶冰冻混合人血清候选标准物质的制备和稳定性

徐静, 张建平, 王清涛

首都医科大学附属北京朝阳医院检验科,北京 100020

收稿日期:2011-11-09 修回日期:1900-01-01 出版日期:2012-04-21 发布日期:2012-04-21
通讯作者: 王清涛

Preparation and stability of the mixed human candidate reference standard serum for gamma-glutamyltransferase

XU Jing, ZHANG Jian-ping, WANG Qing-tao

Department of Laboratory Medical Science, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China

Received:2011-11-09 Revised:1900-01-01 Online:2012-04-21 Published:2012-04-21

摘要/Abstract

摘要： 目的探讨γ-谷氨酰基转移酶(gamma-glutamyltransferase,GGT)冰冻混合人血清候选标准物质制备方法的可行性,同时观察并分析其在-80 ℃保存的稳定性。方法对收集的GGT血清按5个浓度水平进行混合过滤分装,同时分别在0、7、30、60、120、180、330、360、390、750 d测定GGT冰冻混合人血清候选标准物质,采用线性回归方差分析对测定结果进行稳定性检验,评价其稳定性。结果在750 d以内,C1~C5线性回归方差分析的P值均>0.05(C1~C5测定结果P值分别为0.886,0.753,0.585,0.571,0.945)。结论本实验室采用的GGT冰冻混合人血清候选标准物质制备方法简便易行,且候选人血清标准物质在750 d内稳定性良好。

关键词: γ-谷氨酰基转移酶, 标准物质, 稳定性

Abstract: Objective To investigate the feasibility of freezing admixing human serum which was the selected standard materials of gamma-glutamyltransferase(GGT), and to observe and analyze their stability at -80 ℃. Methods The GGT serum which had been collected was mixed, filtered and subpackaged according to five levels. Then the selected standard materials were measured at 0, 7, 30, 60, 120, 180, 330, 360, 390 and 750 days. In order to evaluate the stability, the results were statistic analyzed by linear regression. Results In the 750d, the P of the linear regression about C1~C5 was more than 0.05(the P of C1~C5 was 0.886, 0.753, 0.585, 0.571 and 0.945). Conclusion The preparation method of the freezing admixing human serum which was the selected standard materials of GGT was simple and convenient, otherwise the stability of the selected standard materials was well in 750 days.

Key words: gamma-glutamyltransferase, reference material, stability

中图分类号:

R 446.11

徐静;张建平;王清涛. 探讨γ-谷氨酰基转移酶冰冻混合人血清候选标准物质的制备和稳定性[J]. 首都医科大学学报, 2012, 33(2): 165-168.

XU Jing;ZHANG Jian-ping;WANG Qing-tao. Preparation and stability of the mixed human candidate reference standard serum for gamma-glutamyltransferase[J]. Journal of Capital Medical University, 2012, 33(2): 165-168.

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探讨γ-谷氨酰基转移酶冰冻混合人血清候选标准物质的制备和稳定性

Preparation and stability of the mixed human candidate reference standard serum for gamma-glutamyltransferase

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