叶酸和维生素B12对人神经母细胞瘤株SH-SY5Y细胞的保护作用

doi:10.3969/j.issn.1006-7795.2012.06.014

首都医科大学学报 ›› 2012, Vol. 33 ›› Issue (6): 776-778.doi: 10.3969/j.issn.1006-7795.2012.06.014

• 神经系统疾病的基础与临床研究 • 上一篇下一篇

叶酸和维生素B12对人神经母细胞瘤株SH-SY5Y细胞的保护作用

张景燕, 陈娟, 王蓉, 赵静姝, 吴燕川

首都医科大学宣武医院中心实验室神经变性病教育部重点实验室北京老年病医疗研究中心, 北京 100053

收稿日期:2012-08-02 修回日期:1900-01-01 出版日期:2012-12-21 发布日期:2012-12-21
通讯作者: 王蓉

Protective effect of folic acid and vitamin B12 on human neuroblastoma cell line SH-SY5Y cells

ZHANG Jingyan, CHEN Juan, WANG Rong, ZHAO Jingshu, WU Yanchuan

Central Laboratory, Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases, Ministry of Education, Beijing Geriatric Medical Research Center, Beijing 100053, China

Received:2012-08-02 Revised:1900-01-01 Online:2012-12-21 Published:2012-12-21

摘要/Abstract

摘要： 目的通过观察叶酸和维生素B12对人神经母细胞瘤株SH-SY5Y细胞生长的影响,探讨叶酸和维生素B12对神经细胞的保护作用。方法体外培养SH-SY5Y细胞,分为对照组、叶酸组(1.875 mg/L)、维生素B12组(800 μg/L)、叶酸(1.875 mg/L)+维生素B12(800 μg/L)组,进行细胞形态观察、细胞计数分析以及测定各组细胞的噻唑蓝(thiazolyl blue,MTT)代谢率、乳酸脱氢酶(lactate dehydrogenase,LDH)漏出率。结果与对照组相比,叶酸组、维生素B12组、叶酸+维生素B12组的细胞计数和MTT代谢率升高(P<0.05),LDH漏出率降低(P<0.05),在细胞形态上,叶酸组、维生素B12组、叶酸+维生素B12组明显好于对照组,表现为细胞胞体饱满、存活细胞数目增多、贴壁良好、突起延长。结论叶酸、维生素B12能促进神经细胞生长,降低神经细胞死亡率,从而达到保护神经元的作用。

关键词: 叶酸, 维生素B12, 人神经母细胞瘤株SH-SY5Y

Abstract: Objective To observe the protective effect of folic acid and vitamin B12 on human neuroblastoma cell line SH-SY5Y cell growth in vitro. Methods SH-SY5Y cells cultured in vitro, divided into control group, the folic acid(1.875 mg/L) group, vitamin B12 (800 μg/L) group, folic acid (1.875 mg/L) + vitamin B12(800 μg/L) group. Cell morphology, cell count analysis, thiazolyl blue(MTT) metabolic rate, and lactate dehydrogenase(LDH) leakage rate of each group were detected. Results Compared with the control group, cell count and MTT metabolic rate of folic acid group, vitamin B12 group, folic acid+vitamin B12 group increased(P<0.05), and LDH leakage rate decreased(P<0.05). Compared with the control group, cell morphology were much better in those groups, cell body more plump, the number of surviving cells increased, adherence was good and neurite extension could be found. Conclusion Folic acid and vitamin B12 can promote nerve cell growth, reduce nerve cell death, in order to achieve the protection of neurons.

Key words: folic acid, vitamin B12, human neuroblastoma cell line SH-SY5Y

中图分类号:

R592

张景燕;陈娟;王蓉;赵静姝;吴燕川. 叶酸和维生素B12对人神经母细胞瘤株SH-SY5Y细胞的保护作用[J]. 首都医科大学学报, 2012, 33(6): 776-778.

ZHANG Jingyan;CHEN Juan;WANG Rong;ZHAO Jingshu;WU Yanchuan. Protective effect of folic acid and vitamin B12 on human neuroblastoma cell line SH-SY5Y cells[J]. Journal of Capital Medical University, 2012, 33(6): 776-778.

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叶酸和维生素B12对人神经母细胞瘤株SH-SY5Y细胞的保护作用

Protective effect of folic acid and vitamin B12 on human neuroblastoma cell line SH-SY5Y cells

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