白芍提取物及其有效成分抗氧化活性的研究

doi:10.3969/j.issn.1006-7795.2013.01.023

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (1): 120-125.doi: 10.3969/j.issn.1006-7795.2013.01.023

白芍提取物及其有效成分抗氧化活性的研究

夏颖¹, 殷志爽², 石晨¹, 王桥¹, 宋学英³

1. 首都医科大学化学生物学与药学院实验中心, 北京100069;
2. 北京双鹤药业股份有限公司产品开发部, 北京 100012;
3. 首都医科大学医学实验与测试中心, 北京 100069

收稿日期:2012-05-08 出版日期:2013-02-21 发布日期:2013-02-25
通讯作者: 宋学英 E-mail:songxy@ccmu.edu.cn
基金资助:
北京市中医药科技项目(JJ2008-005)。

Antioxidant activity of the crude extract and the active ingredient extracted from Radix Paeoniae Alba

XIA Ying¹, YIN Zhishuang², SHI Chen¹, WANG Qiao¹, SONG Xueying³

1. Experimental Center, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China;
2. Product Research Development, Beijing Double-Crane Pharmaceutical Company Limited, Beijing 100012, China;
3. Medical Experiment and Testing Center, Capital Medical University, Beijing 100069, China

Received:2012-05-08 Online:2013-02-21 Published:2013-02-25
Supported by:
This study was supported by the Traditional Chinese Medicine Technology Project of Beijing(JJ2008-005).

摘要/Abstract

摘要：

目的考察白芍粗提物及其有效成分五没食子酰基葡萄糖(pentagalloylglucose,PGG)抗氧化活性。方法用乙醇为提取剂超声提取白芍中有效成分PGG;用高速逆流法分离纯化PGG;用高效液相色谱及质谱法分析、鉴定PGG;以维生素C(vitamin c, Vc)为阳性对照,用1,1-二苯基-2-苦基苯肼自由基法(1,1-diphenyl-2-picrylhydrazyl, DPPH)和Fe³⁺ -三吡啶三吖嗪法(ferric reducing-antioxidant power,FRAP)法评价白芍粗提物及PGG的抗氧化活性。结果从白芍中分离纯化得到PGG,其纯度为95.7%。DPPH实验表明,白芍粗提物及PGG对DPPH自由基均有较强的清除作用,其残留率的IC₅₀为PGG<白芍粗提物白芍粗提物>Vc。FRAP实验结果表明,与白芍粗提物及Vc相比较,PGG抗氧化作用最强,尤其在浓度较低(如10 μg/mL)时,其FRAP值大于白芍粗提物并远远大于Vc;当浓度增大时,各物质FRAP值均增大,抗氧化能力增强。但Vc增加得更为显著,在100 μg/mL时,Vc的FRAP值为420.8 μmol/L,已接近白芍粗提物的FRAP值,但仍低于PGG的FRAP值567.1 μmol/L。结论中药白芍中含有抗氧化有效成分PGG,PGG的抗氧化作用远大于阳性对照药Vc,PGG将有可能成为一种潜在的天然高效抗氧化剂,具有广泛的临床应用前景。

关键词: 白芍, 五没食子酰基葡萄糖, 抗氧化作用, 1, 1-二苯基-2-苦基苯肼自由基法, Fe³⁺-三吡啶三吖嗪法

Abstract:

Objective To study antioxidant activity of the crude extract and the active ingredient (pentagalloylglucose, PGG) extracted from Radix Paeoniae Alba.Methods The extracts obtained from Radix Paeoniae Alba by ultrasonic extraction with 50% of ethanol as extracting solvent. High-speed counter-current chromatography (HSCCC) was used for separation and purification of PGG in the crude extract from Radix Paeoniae Alba. PGG was analyzed and identified by HPLC and ESI-MS methods. The antioxidant activity of the crude extract and PGG were evaluated by1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and ferric reducing-antioxidant power(FRAP) assay and vitamin C (Vc) was used as a positive control. Results The purity of PGG was 95.7%, which was separated and purified in the crude extract from Radix Paeoniae Alba. DPPH assay showed that both of crude extract and PGG have strong scavenging effect on DPPH. The residual rates of the IC₅₀ were PGGcrude extract>Vc. FRAP assay showed that the PGG was the strongest antioxidant in comparison with the crude extract and Vc especially at lower concentrations (eg, 10 μg/mL). As the concentration increased, the FRAP values of each sample increased, and antioxidant abilities of samples also increased simultaneously. However, FRAP values of Vc increased more significantly. At the concentration of 100 μg/mL, FRAP value of Vc was 420.8 μmol/L that was very close to the FRAP value of crude extract at the same concentration, but still below FRAP value of PGG of 567.1 μmol/L.Conclusion It was shown that PGG, an ingredient extracted from Chinese medicine Radix Paeoniae Alba, has antioxidant activity. The antioxidant effect of PGG was much higher than that of the positive control Vc. PGG may become an effective anti-oxidant with a wide application for clinical treatment of diseases.

Key words: Radix Paeoniae Alba, pentagalloylglucose, anti-oxidation, 1,1-diphenyl-2-picrylhydrazyl assay, ferric reducing-antioxidant powerassay

中图分类号:

R284.2

夏颖, 殷志爽, 石晨, 王桥, 宋学英. 白芍提取物及其有效成分抗氧化活性的研究[J]. 首都医科大学学报, 2013, 34(1): 120-125.

XIA Ying, YIN Zhishuang, SHI Chen, WANG Qiao, SONG Xueying . Antioxidant activity of the crude extract and the active ingredient extracted from Radix Paeoniae Alba[J]. Journal of Capital Medical University, 2013, 34(1): 120-125.

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白芍提取物及其有效成分抗氧化活性的研究

Antioxidant activity of the crude extract and the active ingredient extracted from Radix Paeoniae Alba

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