帕金森病患者血浆增强α-突触核蛋白寡聚体形成

doi:10.3969/j.issn.1006-7795.2013.06.012

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (6): 840-843.doi: 10.3969/j.issn.1006-7795.2013.06.012

• 帕金森病和卒中诊断与发病机制 • 上一篇下一篇

帕金森病患者血浆增强α-突触核蛋白寡聚体形成

尹娜^1,2, 陈予东^1,2, 李昕^1,2, 杨巍巍^1,2, 李旭冉^1,2, 于顺^1,2

1. 首都医科大学宣武医院神经生物学研究室, 教育部神经变性病重点实验室, 北京 100053;
2. 北京市老年病医疗研究中心分子诊断实验室, 北京 100053

收稿日期:2013-10-18 出版日期:2013-12-21 发布日期:2013-12-13
通讯作者: 于顺 E-mail:yushun103@163.com
基金资助:
国家自然科学基金（81071014）；国家重点基础发展计划（973计划）（2011CB504101）；首都卫生发展科研专项课题（首发2011-1001-01）；国家科技支撑计划课题（2012BAI10B03）；北京市自然科学基金（7102076）。

Increased formation of α-synuclein oligomers in plasma of patients with Parkinson’s disease

YIN Na^1,2, CHEN Yudong^1,2, LI Xin^1,2, YANG Weiwei^1,2, LI Xuran^1,2, YU Shun^1,2

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China;
2. Beijing Geriatric Medical and Clinical Center, Beijing 100053, China

Received:2013-10-18 Online:2013-12-21 Published:2013-12-13
Supported by:
This study was supported by the National Natural Science Foundation of China(81071014);National Basic Research Program of China(973 Program)(2011CB504101);The Capital Health Research and Development Project(Shoufa 2011-1001-01);National Science and Technology Support Program(2012BAI10B03);Natural Science Foundation of Beijing(7102076).

摘要/Abstract

摘要：

目的研究帕金森病（Parkinson’s disease，PD）患者血浆对α-突触核蛋白（α-synuclein，α-Syn）寡聚体形成的影响。方法将重组人α-Syn加入PD和对照血浆中，37℃振荡孵育144 h，免疫印迹方法鉴定α-Syn寡聚体形成情况，ELISA方法分析α-Syn寡聚体形成的相对质量浓度。结果 α-Syn在 PD患者血浆中形成寡聚体含量明显高于对照组。结论 PD患者血浆可促进α-Syn寡聚体形成。

关键词: 帕金森病, 血浆, α-突触核蛋白, 寡聚体

Abstract:

Objective To study the effect of plasma of patients with Parkinson's disease(PD) on the formation of oligomeric α-synuclein (α-Syn). Methods Recombinant α-Syn was incubated in the plasma of PD patients and normal subjects. Immunoblotting analysis was used to identify the formation of α-Syn oligomers. A special ELISA method was used to evaluate the amount of α-Syn oligomers formed in PD and control plasma. Results The amount of α-Syn oligomer formed in the plasma of PD patients was higher than that in control plasma. Conclusion Compared with control plasma, the plasma from PD patients enhanced the oligomerization of α-Syn.

Key words: Parkinson's disease, plasma, α-synuclein(α-Syn), oligomers

中图分类号:

R338

尹娜, 陈予东, 李昕, 杨巍巍, 李旭冉, 于顺. 帕金森病患者血浆增强α-突触核蛋白寡聚体形成[J]. 首都医科大学学报, 2013, 34(6): 840-843.

YIN Na, CHEN Yudong, LI Xin, YANG Weiwei, LI Xuran, YU Shun. Increased formation of α-synuclein oligomers in plasma of patients with Parkinson’s disease[J]. Journal of Capital Medical University, 2013, 34(6): 840-843.

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帕金森病患者血浆增强α-突触核蛋白寡聚体形成

Increased formation of α-synuclein oligomers in plasma of patients with Parkinson’s disease

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