不同年龄段人群蛋白质N-糖基的结构谱型研究

doi:10.3969/j.issn.1006-7795.2014.06.019

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (6): 779-784.doi: 10.3969/j.issn.1006-7795.2014.06.019

不同年龄段人群蛋白质N-糖基的结构谱型研究

刘相佟^1,2, 王友信^1,2, 杨兴华^1,2, 董晶³, 王嵬^2,4, 郭秀花^1,2

1. 首都医科大学公共卫生学院流行病与卫生统计学系, 北京 100069;
2. 临床流行病学北京市重点实验室, 北京 100069;
3. 首都医科大学宣武医院健康体检科, 北京 100053;
4. 埃迪斯科文大学医学科学院, 珀斯6027, 澳大利亚

收稿日期:2014-03-20 发布日期:2014-12-15
通讯作者: 郭秀花 E-mail:guoxiuh@ccmu.edu.cn
基金资助:
国家自然科学基金(81373099,81001281),北京市自然科学基金(7131002)

Comparsion of structure of protein N-glycosylation profiles between age groups

Liu Xiangtong^1,2, Wang Youxin^1,2, Yang Xinghua^1,2, Dong Jing³, Wang Wei^2,4, Guo Xiuhua^1,2

1. Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University, Beijing 100069, China;
2. Beijing Municipal Key Laboratory of Clinical Epidemiology, Beijing 100069, China;
3. Physical Examination Department, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China;
4. School of Medical Sciences, Edith Cowan University, Perth 6027, Australia

Received:2014-03-20 Published:2014-12-15
Supported by:
This study was supported by National Natural Science Fundation of China(81373099, 81001281);Natural Science Fundation of Beijing(7131002).

摘要/Abstract

摘要：

目的分析比较不同年龄人群的血浆N-糖基的结构谱型。方法采用整群抽样,以2009年4至7月在首都医科大学宣武医院进行健康体检的212名居民为研究对象,基于高效液相色谱法进行血浆糖基组检测。将年龄以中位数35为切点分组,不同年龄组的糖基含量比较采用两独立样本t检验或非参数检验。结果 212名研究对象的平均年龄为(37.78±17.89)岁,性别比为0.88。无论男性女性,半乳糖基化水平,≥35岁组二半乳糖聚糖含量较高、双触角非半乳糖聚糖含量较低;在两分支糖基的唾液酸化水平,<35岁组二唾液酸化双触角聚糖含量较高;在唾液酸化水平,≥35岁组单唾液酸含量高、三唾液酸较低;岩藻糖基化位置,≥35岁组核心岩藻糖化聚糖含量高;在分支水平,≥35岁组双触角聚糖含量较高,三触角聚糖和四触角聚糖含量较低(P≤0.03)。结论血浆N-糖基结构可能与年龄有关。

关键词: N-糖基, 结构谱型, 高效液相色谱

Abstract:

Objective To explore difference of plasma N-glycosylation structure between age groups. Methods Based on cluster sampling, 212 residents of a certain community were selected and the serum N-glycans profiles were obtained by high performance liquid chromatography(HPLC).Two independent samples t test or nonparametric tests were used to compare the N-glycosylation structure between age groups divided by 35. Results The average age of the 212 subjects was(37.78±17.89) years, and the sex ratio was 0.88. More digalactosylated glycans, monosialylated glycans, core-fucosylated glycans and biantennary glycans were detected in 35 years or more, whereas more biantennary non-galactosylated glycans, disialylated biantennary glycans, trisialylated glycans and triantennary glycans were seen in younger than 35 years(P≤0.03). Conclusion The N-glycome profile in serum is age dependent. This should be taken into consideration in the development of serum glycome markers.

Key words: N-glycosylation, profiles, HPLC

中图分类号:

R195

刘相佟, 王友信, 杨兴华, 董晶, 王嵬, 郭秀花. 不同年龄段人群蛋白质N-糖基的结构谱型研究[J]. 首都医科大学学报, 2014, 35(6): 779-784.

Liu Xiangtong, Wang Youxin, Yang Xinghua, Dong Jing, Wang Wei, Guo Xiuhua. Comparsion of structure of protein N-glycosylation profiles between age groups[J]. Journal of Capital Medical University, 2014, 35(6): 779-784.

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不同年龄段人群蛋白质N-糖基的结构谱型研究

Comparsion of structure of protein N-glycosylation profiles between age groups

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