α-突触核蛋白与血红蛋白复合物的形成可致线粒体中血红蛋白减低

doi:10.3969/j.issn.1006-7795.2016.05.013

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (5): 621-625.doi: 10.3969/j.issn.1006-7795.2016.05.013

α-突触核蛋白与血红蛋白复合物的形成可致线粒体中血红蛋白减低

杨巍巍^1,2, 李旭冉^1,2, 李昕^1,2, 李旭颖^1,2, 于顺^1,2,3

1. 首都医科大学宣武医院神经生物学研究室, 北京 100053;
2. 帕金森病研究北京重点实验室, 北京 100053;
3. 北京脑重大疾病研究院帕金森病研究所, 北京 100053

收稿日期:2016-01-29 出版日期:2016-10-21 发布日期:2016-10-19
通讯作者: 于顺 E-mail:yushun103@163.com
基金资助:
国家重点基础研究发展计划（2011CB504101），国家自然科学基金（81071014，81371200，81401042），国家科技支撑计划课题（2012BAI10B03），首都卫生发展科研专项课题（2011-4001-01），北京市自然科学基金（7122035）。

Neuronal hemoglobin in mitochondria is reduced by forming a complex with α-synuclein

Yang Weiwei^1,2, Li Xuran^1,2, Li Xin^1,2, Li Xuying^1,2, Yu Shun^1,2,3

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China;
3. Center for Parkinson's Disease, Beijing Insititute for Brain Disorders, Beijing 100053, China

Received:2016-01-29 Online:2016-10-21 Published:2016-10-19
Supported by:
This study was supported by National Basic Research Program ("973" Program) of China (2011CB504101), National Natural Science Foundation of China (81071014, 81371200, 81401042), National Science and Technology Support Program (2012BAI10B03), Capital Health Research and Development Special Fund (2011-4001-01), Natural Science Foundation of Beijing (7122035).

摘要/Abstract

摘要： 目的比较不同年龄段食蟹猴中α-突触核蛋白（α-synuclein，α-syn）与脑血红蛋白（neuronal hemoglobin，nHb）复合物在不同亚细胞中的形成。方法免疫共沉淀（co-immunoprecipitation，Co-IP）及酶联免疫吸附试验（enzyme linked immunosorbent assay，ELISA）检测α-syn与nHb复合物在不同年龄段食蟹猴不同脑区亚细胞结构中的表达，免疫印迹（Western blotting）法检测食蟹猴不同亚细胞结构中游离α-syn与nHb的表达。结果在纹状体中，nHb-α-syn复合物在胞质组分中存在增龄性增加，而在线粒体组分中存在增龄性减低，并且在纹状体中，线粒体中游离nHb随年龄增加而表达减少（P<0.05）；在青、中、老年食蟹猴小脑中，nHb-α-syn复合物在胞质及线粒体组分中并无明显变化。同时，线粒体中游离nHb不随年龄增加发生改变。结论在老化脑的易感区，α-syn与nHb复合物的形成可致线粒体游离nHb降低。

关键词: α-突触核蛋白, 血红蛋白, 老化, 线粒体

Abstract: Objective In this study, we observed whether α-synuclein (α-syn) and neuronal hemoglobin (nHb) formed a complex in different subcellular pool of brain regions in aging cynomolgus monkeys. Methods The levels of nHb-α-syn complex were detected in different subcellular pools of different regions in aging monkey brains using co-immunoprecipitation (Co-IP) and enzyme linked immunosorbent assay (ELISA). The expressions of free α-syn and nHb were detected in different subcellular pools of aging monkey brains by Western blotting. Results In the striatum, cytoplasmic nHb-α-syn complex increased with age, while mitochondrial nHb-α-syn complex decreased with age and was accompanied by a reduction in mitochondrial free nHb (P<0.05). In contrast, no changes in nHb-α-syn complex formation or free nHb levels were detected in the cerebellum in young, middle age and aged monkey brains. Conclusion The above results suggest that α-syn forms a complex with nHb in selected regions of the aging brain.

Key words: α-synuclein (α-syn), hemoglobin, aging, mitochondria

中图分类号:

R338

杨巍巍, 李旭冉, 李昕, 李旭颖, 于顺. α-突触核蛋白与血红蛋白复合物的形成可致线粒体中血红蛋白减低[J]. 首都医科大学学报, 2016, 37(5): 621-625.

Yang Weiwei, Li Xuran, Li Xin, Li Xuying, Yu Shun. Neuronal hemoglobin in mitochondria is reduced by forming a complex with α-synuclein[J]. Journal of Capital Medical University, 2016, 37(5): 621-625.

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α-突触核蛋白与血红蛋白复合物的形成可致线粒体中血红蛋白减低

Neuronal hemoglobin in mitochondria is reduced by forming a complex with α-synuclein

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