紫外线照射未成熟树突状细胞诱导小鼠同种抗原免疫耐受及其机制研究

doi:10.3969/j.issn.1006-7795.2017.03.020

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (3): 431-438.doi: 10.3969/j.issn.1006-7795.2017.03.020

紫外线照射未成熟树突状细胞诱导小鼠同种抗原免疫耐受及其机制研究

郭轶先¹, 张蓝方¹, 孙雪静¹, 万岁桂¹, 夏长青^1,2

1. 首都医科大学宣武医院血液科, 北京 100053;
2. 佛罗里达大学医学院病理学、免疫学和实验医学系, 佛罗里达 32610

收稿日期:2016-01-15 出版日期:2017-05-21 发布日期:2017-06-14
通讯作者: 夏长青 E-mail:bjxwgyx@163.com
基金资助:
国家自然科学基金（81172854，81240015）。

Alloantigen immune tolerance and its mechanism in mice immunized with the ultraviolet radiation immature dendritic cells

Guo Yixian¹, Zhang Lanfang¹, Sun Xuejing¹, Wan Suigui¹, Xia Changqing^1,2

1. Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Florida 32610, USA

Received:2016-01-15 Online:2017-05-21 Published:2017-06-14
Supported by:
This study was supported by National Natural Science Foundation of China (81172854, 81240015).

摘要/Abstract

摘要： 目的探讨应用紫外线照射未成熟树突状细胞诱导C3H小鼠对Balb/c小鼠同种抗原免疫耐受及其免疫学机制。方法 ①用紫外线B（ultraviolet B，UVB）照射Balb/c（H-2d）小鼠骨髓来源的未成熟树突状细胞（immature dendritic cells，imDC），诱导其凋亡，随后将其输注到C3H（H-2k）小鼠体内，诱导C3H小鼠对Balb/c抗原免疫耐受。②检测诱免疫耐受小鼠体内调节T细胞以及细胞因子白细胞介素-10（interleukin-10，IL-10）和干扰素-γ（interferon-γ，IFN-γ）分泌以研究其耐受机制。结果 ①UVB-Balb/c imDC免疫的C3H小鼠对Balb/c抗原产生了免疫耐受，不能产生抗Balb/c抗体。②免疫耐受C3H小鼠不能排异注射到体内的Balb/c脾细胞。③UVB-Balb/c imDC免疫的C3H小鼠T细胞分泌IL-10增加，并且产生更多的FOX-P3⁺调节T细胞。结论 ①应用UVB-Balb/c imDC免疫C3H小鼠可以使C3H小鼠对Balb/c抗原产生完全彻底的免疫耐受。②免疫耐受C3H小鼠对Balb/c抗原产生免疫耐受的可能原因是由于其T细胞分泌IL-10增加，并且产生更多的FOX-P3⁺调节T细胞。

关键词: 树突细胞, 凋亡, 调节T细胞, 免疫耐受

Abstract: Objective To induce alloantigen immune tolerance between C3H mice and Balb/c mice by infusing the ultraviolet B(UVB) radiation immature dendritic cells (imDC) and and study the immunological mechanisms of this process.Methods ①The authors induced alloantigen tolerance in C3H mice (H-2k) by intravenous injecting ultraviolet B (UVB) irradiated Balb/c immature dendritic cells (UVB-Balb/c imDC) derived from the cultures of Balb/c bone marrow cells. ②Detection of immune tolerance induced regulatory T cells, interleukin-10 (IL-10) and interferon-γ (IFN-γ) in mice in order to study the tolerance mechanism. Results ①C3H mice immunized with UVB-Balb/c imDC can produce immune tolerance to Balb/c antigens and can not produce anti-Balb/c antibody. ②C3H mice immunized with UVB-Balb/c imDC can not clear the Balb/c spleen cells in vivo. ③The T cells of C3H mice immunized with UVB-Balb/c imDC can secrete more IL-10 and produce more FOX-P3⁺ regulatory T cells than the control group in vitro. Conclusion ①Immunization of C3H mice with UVB-Balb/c imDC allows C3H mice to fully tolerate Balb/c antigen. ②The possible cause of immune tolerance to Balb/c antigens in C3H mice is due to the increased secretion of IL-10 from T cells and the production of more FOX-P3⁺regulatory T cells.

Key words: dendritic cells, apoptosis, regulatory T cells, immune tolerance

中图分类号:

郭轶先, 张蓝方, 孙雪静, 万岁桂, 夏长青. 紫外线照射未成熟树突状细胞诱导小鼠同种抗原免疫耐受及其机制研究[J]. 首都医科大学学报, 2017, 38(3): 431-438.

Guo Yixian, Zhang Lanfang, Sun Xuejing, Wan Suigui, Xia Changqing. Alloantigen immune tolerance and its mechanism in mice immunized with the ultraviolet radiation immature dendritic cells[J]. Journal of Capital Medical University, 2017, 38(3): 431-438.

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紫外线照射未成熟树突状细胞诱导小鼠同种抗原免疫耐受及其机制研究

Alloantigen immune tolerance and its mechanism in mice immunized with the ultraviolet radiation immature dendritic cells

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