储存条件对尿触珠蛋白检测的影响

doi:10.3969/j.issn.1006-7795.2020.01.004

首都医科大学学报 ›› 2020, Vol. 41 ›› Issue (1): 21-26.doi: 10.3969/j.issn.1006-7795.2020.01.004

• 糖尿病的临床与基础研究 • 上一篇下一篇

储存条件对尿触珠蛋白检测的影响

郑晓敏^1,2,3,4, 杨芳远^2,3,4, 刘翠平¹, 杨金奎^2,3,4

1. 清华大学附属垂杨柳医院内分泌科, 北京 100022;
2. 首都医科大学附属北京同仁医院内分泌科, 北京 100730;
3. 糖尿病防治研究北京市重点实验室, 北京 100730;
4. 北京市糖尿病研究所, 北京 100730

收稿日期:2019-12-12 出版日期:2020-02-21 发布日期:2020-02-13
通讯作者: 杨金奎 E-mail:jinkui.yang@gmail.com
基金资助:
国家重点研发项目（2017YFC0909600），国家自然科学基金（81561128015），北京市科委科技计划项目（D171100002817005）。

Effects of storage condition on measurement of haptoglobin in urine

Zheng Xiaomin^1,2,3,4, Yang Fangyuan^2,3,4, Liu Cuiping¹, Yang Jinkui^2,3,4

1. Department of Endocrinology, Chuiyangliu Hospital affiliated to Tsinghua University, Beijing 100022, China;
2. Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
3. Beijing Key Laboratory of Diabetes Research and Care, Beijing 100730, China;
4. Beijing Diabetes Institute, Beijing 100730, China

Received:2019-12-12 Online:2020-02-21 Published:2020-02-13
Supported by:
This study was supported by National Key R&D Program of China(2017YFC0909600),National Natural Science Foundation of China(81561128015),Beijing Municipal Science & Technology Commission (D171100002817005).

摘要/Abstract

摘要： 目的研究不同标本处理方法及储存条件对尿触珠蛋白（haptoglobin，Hp）检测的影响。方法收集40例2型糖尿病患者的尿标本，分别进行离心及不离心处理后于室温或4℃放置3、6和24 h，采用ELISA法检测尿Hp浓度。结果未经离心处理的标本，室温放置3 h和6 h尿Hp浓度较基础值无明显变化（P均>0.05），而24 h尿Hp浓度显著下降（P < 0.01），4℃储存放置3 h较基础值无明显变化（P>0.05），但6 h尿Hp浓度明显下降（P < 0.01）。离心标本在室温和4℃放置3 h稳定（P均>0.05），放置6 h尿Hp浓度明显下降（P均< 0.01）。结论室温放置6 h的未离心标本可保持尿Hp浓度稳定。冷藏及离心可导致尿Hp浓度的降低。

关键词: 触珠蛋白, 糖尿病肾脏疾病, 储存

Abstract: Objective To investigate the effects of different treatments of urinary specimen and storage conditions on urinary haptoglobin levels. Methods The urinary samples of 40 patients with type 2 diabetes were stored at room temperature and 4℃ for 3 hours, 6 hours and 24 hours with and without centrifugation, and then the levels of urinary haptoglobin were measured with enzyme-linked immunosorbent assay. Results For the samples without centrifugation, no change in urinary haptoglobin concentration was observed after 3 hours and 6 hours of room temperature storage(both P>0.05), but there was a significantly decrease after 24 hours(P < 0.01). There was no statistically significant difference in the levels of urinary haptoglobin after 3 hours at 4℃ (P>0.05), and there was a significantly decrease after 6 hours(P < 0.01). The centrifuged samples were stable for 3 hours at both room temperature and 4℃(both P>0.05), but there was a significantly decrease after 6 hours(both P < 0.01).Conclusion It was recommended to store urine samples at room temperature when the measurement of urinary haptoglobin could be completed within 6 hours. Cold storage and centrifugation resulted in the decrease of urinary haptoglobin level.

Key words: haptoglobin, diabetic kidney disease, storage

中图分类号:

R587.2

郑晓敏, 杨芳远, 刘翠平, 杨金奎. 储存条件对尿触珠蛋白检测的影响[J]. 首都医科大学学报, 2020, 41(1): 21-26.

Zheng Xiaomin, Yang Fangyuan, Liu Cuiping, Yang Jinkui. Effects of storage condition on measurement of haptoglobin in urine[J]. Journal of Capital Medical University, 2020, 41(1): 21-26.

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储存条件对尿触珠蛋白检测的影响

Effects of storage condition on measurement of haptoglobin in urine

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