气相色谱法测定柑橘类果皮挥发油柠檬烯含量的方法研究

doi:10.3969/j.issn.1006-7795.2021.03.017

首都医科大学学报 ›› 2021, Vol. 42 ›› Issue (3): 443-446.doi: 10.3969/j.issn.1006-7795.2021.03.017

气相色谱法测定柑橘类果皮挥发油柠檬烯含量的方法研究

邵建群, 李青原, 李风华, 石晨, 王玉记, 张枫^*

首都医科大学药学院实验教学中心,北京 100069

收稿日期:2020-06-30 出版日期:2021-06-21 发布日期:2021-06-16
通讯作者: *E-mail:zhangf@ccmu.edu.cn

Determination of limonene in volatile oil from citrus peel by gas chromatography

Shao Jianqun, Li Qingyuan, Li Fenghua, Shi Chen, Wang Yuji, Zhang Feng^*

Experimental Teaching Center, School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China

Received:2020-06-30 Online:2021-06-21 Published:2021-06-16
Contact: *E-mail:zhangf@ccmu.edu.cn

摘要/Abstract

摘要： 目的建立柑橘类果皮挥发油测定柠檬烯含量的方法。方法采用水蒸气蒸馏法提取柑橘果皮中的挥发油,气相色谱法测定挥发油中柠檬烯含量。色谱条件:色谱柱HP-5(30 m×0.32 mm×0.25 μm);氢火焰离子化检测器(flame inoization detector, FID);进样口温度250 ℃;检测器温度250 ℃。程序升温:起始温度70 ℃,保留16 min,以30 ℃/min升温至250 ℃,保留1 min。载气氮气的流速1 mL/min;分流比100∶1,进样量1 μL。结果柠檬烯在0~17.6 mg/mL范围内与峰面积有良好的线性关系(r²=0.999 0),平均加样回收率为103.4%(n=9),峰面积相对标准偏差(relative standard deviation,RSD)为2.10%(n=9)。结论本实验方法重复性好、灵敏度和准确性高,可用于柑橘类果皮挥发油中柠檬烯含量的评价方法。

关键词: 气相色谱, 柑橘果皮, 挥发油, 柠檬烯, 含量测定

Abstract: Objective To establish a method for the content determination of limonene in volatile oil from citrus peels. Methods The volatile oil was extracted by steam distillation and the content of limonene was determined by gas chromatography. Gas chromatography conditions were as follows: the capillary column was HP-5(30 m×0.32 mm×0.25 μm)with hydrogen flame ionization detector (FID), and the temperature of inlet and detector were 250 ℃. The temperature program of colum oven started at 70 ℃, holding for 16 minutes, then increased to 250 ℃ at a rate of 30 ℃·min^-1, kept for 1 min. The carrier gas was nitrogen with flow rate of 1 mL·min^-1. Split ration was 100∶1, and injection volume was 1 μL. Results Limonene was linear in the range of 0-17.6 mg·mL^-1(r²=0.999 0). The average recovery was 103.4%(n=9), and relative standard deviation (RSD) was 2.10%(n=9). Conclusion The experiment method has good repeatability, high sensitivity and accuracy, which can be used to determine the content of limonene and control quality in volatile oil from citrus peel.

Key words: gas chromatography, citrus peels, volatile oil, limonene, content determination

中图分类号:

R284

邵建群, 李青原, 李风华, 石晨, 王玉记, 张枫. 气相色谱法测定柑橘类果皮挥发油柠檬烯含量的方法研究[J]. 首都医科大学学报, 2021, 42(3): 443-446.

Shao Jianqun, Li Qingyuan, Li Fenghua, Shi Chen, Wang Yuji, Zhang Feng. Determination of limonene in volatile oil from citrus peel by gas chromatography[J]. Journal of Capital Medical University, 2021, 42(3): 443-446.

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气相色谱法测定柑橘类果皮挥发油柠檬烯含量的方法研究

Determination of limonene in volatile oil from citrus peel by gas chromatography

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