首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (1): 113-117.doi: 10.3969/j.issn.1006-7795.2014.01.024

• 基础研究 • 上一篇    下一篇

荧光分析法测定元宝枫叶中总黄酮含量的研究

马登磊1,2, 邵建群1, 何深知1, 张枫1   

  1. 1. 首都医科大学化学生物学与药学院实验教学中心, 北京 100069;
    2. 首都医科大学宣武医院药物研究室, 北京 100053
  • 收稿日期:2013-09-19 出版日期:2014-02-21 发布日期:2014-02-21
  • 通讯作者: 张枫 E-mail:zhangf@ccmu.edu.cn
  • 基金资助:

    北京市教委实验室建设-药学实验中心的实验室建设（PXM2013-014226-07-000025），北京市教委人才培养模式创新试验区-创新型药学人才培养试验区（PXM2013-014226-07-000001）。

Determination of total flavonoid in leaves of acer truncatum Bunge by spectrofluorimetry

Ma Denglei1,2, Shao Jianqun1, He Shenzhi1, Zhang Feng1   

  1. 1. Experimental Teaching Center, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China;
    2. Department of Pharmacology, Xuan wu Hospital, Capital Medical University, Beijing 100053, China
  • Received:2013-09-19 Online:2014-02-21 Published:2014-02-21
  • Supported by:

    This study was supported by the Beijing Municipal Education Commission for the Laboratory Construction of Pharmacy Experimental Center (PXM2013-014226-07-000025);Pharmacy Talent Training Mode Innovation Test Site (PXM2013-014226-07-000001).

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177

被引次数

19

摘要：

目的 采用荧光分析方法，对元宝枫叶总黄酮含量进行测定研究。方法 以芦丁为标准对照品，利用黄酮与Al3+形成稳定荧光络合物的性质，选择激发波长λex=470 nm，发射波长λem=547 nm，研究乙醇的体积分数、5%Al（NO3）3的加入量、pH、放置时间以及绿原酸对荧光强度的影响。结果 最佳测定条件是以60%乙醇溶液作为溶剂，在10 mL的容量瓶中加入1 mL 5% Al（NO3）3，并使测定溶液pH=5。该方法测定芦丁质量浓度在6.55×10-6mol/L~3.20×10-5mol/L与荧光强度具有良好的线性关系，且回归方程为y=1.147 7x + 0.430 4，R2=0.999 1。结论 采用荧光分析法，通过改变激发光的波长，能够减小绿原酸对黄酮测定的干扰，可作为元宝枫叶总黄酮含量测定的参考方法。

关键词: 元宝枫叶, 总黄酮, 含量测定, 荧光分析法

Abstract:

Objective To propose a method to determine the total flavonoid in leaves of Acer truncatum Bunge by Spectrofluorimetry. Methods Based on fluorescent property that flavonoids can make a stable complexation with Al3+, the excitation and emission wavelengths were selected at 470 nm and 547 nm respectively with rutin as the standard sample to research effects of solvent, the dosage of 5%Al(NO3)3 solution, pH, standing time and chlorogenic acid. Results The optimum conditions were 60% ethanol as solvent, added 1 mL of 5%Al(NO3)3 solution and made pH=5 in 10 mL volumetric flask. The fluorescence intensity had a good linear relationship with rutin concentration in the range of 6.55×10-6mol/L~3.20×10-5mol/L. The regression equation was found to be y=1.147 7x + 0.430 4,R2=0.999 1. Conclusion This method could lower the influence of chlorogenic acid for determination total flavonoid by changing the excitation wavelength with fluorescence analysis and the work provided a reference for quantitative analysis of total flavonoid in the leaves of Acer truncatum Bunge.

Key words: leaves of Ace truncatum Bunge, total flavonoid, determination, spectrofluorimetry

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