首都医科大学学报 ›› 1992, Vol. 13 ›› Issue (2): 92-96.

• 论著 • 上一篇    下一篇

氧化过氧化反应结合法显示单胺氧化酶阳性神经元的研究

鲁强, 赵春礼, 张国风   

  1. 首都医学院北京市神经科学研究所
  • 收稿日期:1991-02-28 修回日期:1900-01-01 出版日期:1992-04-15 发布日期:1992-04-15

The Study on the Demonstration of Monoamine Oxidase-containing Positive Neurons by Coupled Peroxidatic Oxidation

Lu Qiang, Zhao Chunli, Zhang Guofeng   

  1. Beijing Institute for Neuroscience, Capital Institute of Medicine
  • Received:1991-02-28 Revised:1900-01-01 Online:1992-04-15 Published:1992-04-15

摘要: 以氧化过氧化反应结合法显示中枢神经系统内单胺氧化酶(MAO)阳性神经元,在显示胞体、突起和纤维走行的清晰程度上优于四唑盐法。利用Clorgyline和Deprenyl分别抑制A型和B型MAO,可以选择性显示两种不同类型MAO在中枢的分布。本研究还探讨了孵育液内HRP和盐酸酪胺浓度与呈色反应的关系。

关键词: 单胺氧化酶, 组织化学, 抑制剂

Abstract: The method of coupled peroxidatic oxidation was proved to be prior to the tetazolium salts one is showing the clarity of the somata, process and fibre courses of monoamine oxi-dase(MAO)-contining positive neruons in the cental nerous system. The clistibution of two different types of MAO A and MAO B in the central nervous system could be selectively demonsated when the MAO A and MAO B were supressed by deprenyl and clorgyline, re-spectively. The relationship between concentation of tyranine or HRP in the medium and chomatic reaction was also discussed in the present study.

Key words: monoamine oxidase, histochemisty, inhibitor

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