首都医科大学学报 ›› 1993, Vol. 14 ›› Issue (1): 44-48.

• 论著 • 上一篇    下一篇

血管紧张素Ⅰ转换酶抑制剂八肽对大鼠的降压作用及与脑钠素之间的关系

张万江1, 盛树力1, 韩劲勇1, 艾厚喜1, 胡晓愚2, 郭春远1, 赵志炜1   

  1. 1. 北京市老年病医疗研究中心 首都医学院宣武医院;2. 兰州大学
  • 收稿日期:1991-11-21 修回日期:1900-01-01 出版日期:1993-01-15 发布日期:1993-01-15

Depnesive Action of Angiotensin-converting Enzyme Inhibitor Octopeptide on Blood Pressure and Its Relationship with Brain Natriuretic Peptide in Rats

Zhang Wanjiang1, Sheng Shuli1, Han Jinyong1, Ai Houxi1, Hu Xiaoyu2, Guo Chunyuan1, Zhao Zhiwei1   

  1. 1. Beijing Geriatric Center, Beijing Xuanwu Hospital, Capital Institute of Medicene;2. Lanzhou University
  • Received:1991-11-21 Revised:1900-01-01 Online:1993-01-15 Published:1993-01-15

摘要:

用固相法在国际上首次合成了高纯度并具有生物活性的 ACEI 八肽,并对其生物活性进行了研究。研究结果表明;①ACEI 八肽对人血和脑组织及大鼠血和肺组织的血管紧张素转换酶(ACE)具有明显的抑制作用。②ACEI 八肽静脉快速注入(60μg/kg)可明显降低大鼠血压及降低血醛固酮浓度。侧脑室(icy)注射(6μg/kg)对血压没有影响。③少量 ACEI 八肽在不影响血管紧张素和醛固酮浓度的情况下能加强脑钠素(BNP)的降压效果。④BNP 或其片断具有抑制 ACE 活性的作用。结果对于肾素-血管紧张素系统特别是高血压病生理的深入研究具有重要意义。

关键词: 血管紧张素 I 转换酶, 血管紧张素 I 转换酶抑制剂八肽, 化学合成, 脑钠素, 血压

Abstract:

We first reported in literature the syuthesize of high purity and biologically active ACEI oetapeptide with solid phase method.The resulta of the study on the relationship between depressive action on blood pressure of ACEI and brain natiuretic peptide(BNP)showed that: 1)ACEI octopeptide inhibited significantly ACE level of plasma and membrane fraction of lung tissue in rats.IC50 was 10μmol/L and 5 μmol/L respectively;2)although ACEI admin-istrated(60 μg/0.6 ml/kg)by iv rapidly could supress blood pressure and aldosterone con-centration of plasma in rats,icy injection of ACEI(6μg/0.6ml.kg-)was found no effect on blood pressure;3)tiny amount of ACEI octapeptid with no influence on plasma AT-Ⅰand aldosterone concentration increased the hypotensive effect of BNP,and 4)BNP or its fragments was found inhibitive on human plasma ACE activity.We conclude that our result may play an important role in the studies of renin-angioleusin system and physio-pathology of hyperteusive diseases.

Key words: angiotensin converting enzyme, angiotensin converting enzyme inhibitor octopeptide, brain natfiuretic peptide, blood pressure

中图分类号: