首都医科大学学报 ›› 1994, Vol. 15 ›› Issue (2): 89-91.

• 论著 • 上一篇    下一篇

缺血心肌切片琥珀酸脱氢酶组织化学染色的光密度定量研究

温祥云1, 陈铁军1, 吕福东1, 温嘉2   

  1. 1. 首都医学院病理解剖学教研室;2. 中国医学科学院生物技术研究所
  • 收稿日期:1993-05-07 修回日期:1900-01-01 出版日期:1994-04-15 发布日期:1994-04-15

A Quantitalive Study on the Optical Density of Succinate Dehydrogenase Histochemical Staining of Ischemic Myocardial Sections

Wen Xiangyun1, Chen Tiejun1, Lü Fudong1, Wen Jiayu2   

  1. 1. Department of Pathology, Captial Institute of Medicine;2. Biotechnique Institute, Chinese Academy of Medical Sciences
  • Received:1993-05-07 Revised:1900-01-01 Online:1994-04-15 Published:1994-04-15

摘要: 用分光光度计对缺血心肌切片琥珀酸脱氢酶组织化学反应强度进行光密度定量观察.结果显示,缺血15min后,酶活性即明显降低,提高了单纯形态学诊断的敏感性.

关键词: 心肌缺血, 琥珀酸脱氢酶, 光密度

Abstract: The optical density measurement of the succinate dehydrogenase histochemical staining sections of ischemic myocardium with 680 nm of spectrophotometer was made. The result indicated that if the duration of ischemia was more than 15 min,the enzymatic activity showed prominent decrease.This method raised definitely the diagnostic sensitivity for succinate dehydrogenase activity as compared with the mere morphological method.

Key words: myocardial ischemia, succinate dehydrogenase, optical density

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