新城疫弱病毒对人肺腺癌细胞系A549多细胞球体的作用

首都医科大学学报 ›› 1997, Vol. 18 ›› Issue (3): 209-213.

新城疫弱病毒对人肺腺癌细胞系A549多细胞球体的作用

潘李珍

首都医科大学细胞室

收稿日期:1996-11-21 修回日期:1900-01-01 出版日期:1997-07-15 发布日期:1997-07-15

Effects of Newcastle Disease Virus(Losota-4) on Human Lung Adenocarcinoma Cell Line A549 Multicellular Spheriods

Pan Lizhen

Department of Cell Biology, Capital University of Medical Sciences

Received:1996-11-21 Revised:1900-01-01 Online:1997-07-15 Published:1997-07-15

摘要/Abstract

摘要：

用人肺腺癌细胞系A549多细胞球体研究了新城疫弱病毒(NDV)的抗癌细胞增殖作用。实验证明使用各种NDV稀释液(1/20、1/100、1/500)作用于球体3d,均能使球体延缓生长,与对照组相比差异显着(P<0.05).球体表面生长单层细胞能力的测定证实,用NDV1/20、1/100稀释液处理后的球体,可以在24孔盘中贴壁,但不能向外生长细胞,球体萎缩。NDV1/500稀释液作用后的球体能贴壁,但只向外生长部分细胞。对照组球体接种后立即贴壁,并向外生长大片细胞,形成较大的生长晕。

关键词: 新城疫弱病毒, 肺腺癌, 多细胞球体

Abstract:

The cytotoxic effects of NDV(Losota-4)were investigated on multicellular spheriods of human lung adenocarcinoma A549.Spheriods of A549 were cultured at 37 ℃,5% CO₂ for three days in various titers of NDVranging from 1/20,1/100 to 1/500 and then the diameters of sphriods were counted and cell proliferation from spheriods and their adhesion to culture surface were observed. The results indicated that the mean diameter of spheriods was decreased in all titers (P<0.05). Titers of NDVfrom 1/20 to 1/100 can inhibit cell proliferation from A549 spheriods and adhesion to culture surfaces.

Key words: Newcastle disease virus, human lung adenocarcinoma, multicellular spheriods

中图分类号:

R329.28

潘李珍. 新城疫弱病毒对人肺腺癌细胞系A549多细胞球体的作用[J]. 首都医科大学学报, 1997, 18(3): 209-213.

Pan Lizhen. Effects of Newcastle Disease Virus(Losota-4) on Human Lung Adenocarcinoma Cell Line A549 Multicellular Spheriods[J]. Journal of Capital Medical University, 1997, 18(3): 209-213.

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