淋病奈瑟菌PCR检测的临床应用研究

首都医科大学学报 ›› 1999, Vol. 20 ›› Issue (1): 37-39.

淋病奈瑟菌PCR检测的临床应用研究

张景生, 肖白, 谭淑珍, 严敬丽, 蔡有龄, 刘敬忠

首都医科大学附属北京红十字朝阳医院皮肤科

收稿日期:1988-03-25 修回日期:1900-01-01 出版日期:1999-01-15 发布日期:2013-10-16

Clinical Application of PCR Assay for Detection of Neisseria gonorrhoeae

Zhang Jingsheng, Xiao Bai, Tan Shuzhen, Yan Jingli, Cai Youling, Liu Jingzhong

Department of Dermatology, Beijing Red Cross Chaoyang Hospital, Affiliate of Capital University of Medical Sciences

Received:1988-03-25 Revised:1900-01-01 Online:1999-01-15 Published:2013-10-16

摘要/Abstract

摘要： 为评价淋病奈瑟菌PCR检测的临床效果,建立了对标准淋病奈瑟菌的DNA PCR检测技术。结果显示,NG-PCR技术的灵敏度约为3个菌的基因组拷贝。对8种菌株进行NG-PCR,只有标准淋病奈瑟菌显示阳性,其余7株非淋球菌菌株均阴性,说明有很好的特异性。对49例淋病患者同时进行涂片镜检法、细菌培养法和PCR法检测的比较,其阳性检出率分别为76.6%、52.2%和95.9%.提示NG-PCR方法比涂片和培养法更为敏感,且特异性强,在淋病的诊断,特别是早期诊断中有重要参考价值。

关键词: 淋病奈瑟菌, 聚合酶链反应, 临床应用

Abstract: Inder to evaluate the clinical application of PCR assay for the detection of Neisseria gonorrhoeae. Genitourinary specimens were obtained from NG patients and Neisseria gonorrhoeae was detected with a number of tests including Gram's stain of smears, culture and PCRassay. The sensitivity and specificity of the NG-PCR assay were evaluated with 8 bacterial strains. The NG-PCR assay had a detection limit of 3 copies of NG genomic DNA. In the specificity of the PCRassay, the standard Neisseria gonorrheae was positive, but no PCR product could be visualized in 7 nongonorrhoeae strains. PCRresults of 47 NG patients were compared with those obtained by Gram's smear and culture. The positive rates for smear, culture and PCRwere 76.6%, 52.2% and 95.9%, respectively. Of 82 normal controls, 1 specimen was PCR positive. The false positive rate of the PCRassay was about 1.2%. The PCR assay is a satisfactory diagnostic tool for the detection of Neisseria gonorrhoeae in clinical specimens.

Key words: Neisseria gonorrhoeae, PCR, clinical applying

中图分类号:

R759.22

张景生;肖白;谭淑珍;严敬丽;蔡有龄;刘敬忠. 淋病奈瑟菌PCR检测的临床应用研究[J]. 首都医科大学学报, 1999, 20(1): 37-39.

Zhang Jingsheng;Xiao Bai;Tan Shuzhen;Yan Jingli;Cai Youling;Liu Jingzhong. Clinical Application of PCR Assay for Detection of Neisseria gonorrhoeae[J]. Journal of Capital Medical University, 1999, 20(1): 37-39.

[1]	方芳, 陈柯霖, 陈燕, 黄泽玉, 吕虹, 刘淑静, 周金, 张国军, 康熙雄. 应用CNAS CL-36标准评估HBV DNA定量检测试剂的抗干扰能力[J]. 首都医科大学学报, 2016, 37(4): 495-499.
[2]	薛奋勤, 许晴, 魏华, 李华, 薛冰. 仅采用PCR进行分子克隆的方法探索[J]. 首都医科大学学报, 2014, 35(6): 785-789.
[3]	何婧瑜, 华琦. 乌拉地尔在心血管疾病中的临床应用[J]. 首都医科大学学报, 2013, 34(1): 100-104.
[4]	吕虹;高志勇;张国军;闫惠平;康熙雄. 诺如病毒性胃肠炎快速检测方法的研究[J]. 首都医科大学学报, 2012, 33(2): 148-152.
[5]	向绍通;徐书雯;李东风. 迟发性阿尔茨海默病患者血清尿激酶型纤溶酶原激活因子浓度的测定及其意义[J]. 首都医科大学学报, 2012, 33(1): 103-106.
[6]	孙秀静;朱圣韬;徐有青;张澍田. SYBRGreenⅠ实时荧光定量PCR检测人c-myc基因方法的建立[J]. 首都医科大学学报, 2009, 30(6): 821-826.
[7]	向绍通;徐书雯;李东风. 尿激酶型纤溶酶原激活因子基因P141L多态性与迟发性阿尔茨海默病的关系[J]. 首都医科大学学报, 2008, 29(3): 278-282.
[8]	金建敏;张沪生;陈东宁. 嗜肺军团菌与非嗜肺军团菌所致社区获得性肺炎的临床对比分析[J]. 首都医科大学学报, 2008, 29(3): 348-353.
[9]	关键;王国干;朱俊. 直接凝血酶抑制剂及其临床应用[J]. 首都医科大学学报, 2007, 28(4): 462-466.
[10]	季秀娟;徐剑;张雪莲;郭丽敏;杨金奎. 血管紧张素转化酶相关性羧肽酶在人及啮齿动物胰腺的表达[J]. 首都医科大学学报, 2007, 28(3): 278-282.
[11]	温艳;阴赪宏;黄敏君;栗绍刚;李威;王非. PCR诊断细菌性痢疾的临床价值[J]. 首都医科大学学报, 2006, 27(2): 171-175.
[12]	石琳;申阿东;李晓峰;柏松;关晓蕾;李仲智. 中国先天性心脏病Nkx2.5基因突变筛查及关联研究[J]. 首都医科大学学报, 2005, 26(5): 525-528.
[13]	樊剑锋;杨永弘;马琳;佟月娟. 耐甲氧西林金黄色葡萄球菌抗生素耐药和耐药基因的检测[J]. 首都医科大学学报, 2005, 26(5): 540-544.
[14]	周兵;张伟;孙文艳;陶格陶呼;杨凌. 用PCR法检测真菌性鼻窦炎分泌物中的真菌[J]. 首都医科大学学报, 2005, 26(3): 249-251.
[15]	褚金花;宣登峰;吴萍;张淑华;李国君;耿荣. 线粒体直接PCR扩增法[J]. 首都医科大学学报, 2004, 25(3): 284-285.