缺氧预适应小鼠脑组织中缺氧诱导因子-1的表达

首都医科大学学报 ›› 2001, Vol. 22 ›› Issue (4): 285-287.

缺氧预适应小鼠脑组织中缺氧诱导因子-1的表达

梁元晶, 吕国蔚

首都医科大学神经生物学系

收稿日期:2001-02-20 修回日期:1900-01-01 出版日期:2001-10-15 发布日期:2001-10-15

Expression of Hypoxia Inducible Factor-1 in the Brain of Hypoxia Preconditioned Mouse

Liang Yuanjing, Lü Guowei

Department of Neurobiology, Capital University of Medical Sciences

Received:2001-02-20 Revised:1900-01-01 Online:2001-10-15 Published:2001-10-15

摘要/Abstract

摘要： 为探讨缺氧诱导因子-1(HIF-1)在缺氧预适应小鼠脑组织中的表达,用Western印迹法检测慢性缺氧(H)和不缺氧(C)HeLa细胞、急性重复性缺氧0次(H₀)、1次(H₁)、4次(H₄)小鼠脑组织中的HIF-1α.结果:慢性缺氧HeLa细胞中可检测到较多的HIF-1α,H₀组脑组织中检测不到HIF-1α,H₁组检测到微量HIF-1α,H₄组检测到较多HIF-1α.结果显示:脑组织中HIF-1α 的含量随缺氧预适应的产生而逐步增加,提示HIF-1可能参与缺氧预适应的形成.

关键词: 缺氧诱导因子-1, 缺氧预适应, Western印迹法

Abstract: Hypoxia inducible factor -1(HIF-1)is a transcriptional factor induced when mammalian cells are exposed to chronic hypoxia. In this study, chronic hypoxic(H)and nomorxic(C)HeLa cells, brain tissue without hypoxia exposure(H₀), that with 1 run of hypoxia exposure(H₁)and with 4 runs of hypoxia exposure(H₄)were collected. Cellular extract were prepared and analyzed by Western blot assay to detect HIF-1α. HIF-1α could be detected in chronic hypoxic HeLa cells. No HIF-1α could be found in group H₀, while a small amount of HIF-1α and much more HIF-1α could be found in group H₁ and H₄ respectively. These results indicate that HIF-1α in brain cells increases during hypoxia preconditioning. The increase of HIF-1 is a possible mechanism for hypoxia preconditioning.

Key words: hypoxia inducible factor-1, hypoxia preconditioning, Western blot assay

中图分类号:

Q 786

梁元晶;吕国蔚. 缺氧预适应小鼠脑组织中缺氧诱导因子-1的表达[J]. 首都医科大学学报, 2001, 22(4): 285-287.

Liang Yuanjing;Lü Guowei . Expression of Hypoxia Inducible Factor-1 in the Brain of Hypoxia Preconditioned Mouse[J]. Journal of Capital Medical University, 2001, 22(4): 285-287.

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