不同培养基体外培养旋毛虫新生蚴成活率分析

首都医科大学学报 ›› 2002, Vol. 23 ›› Issue (3): 206-207.

不同培养基体外培养旋毛虫新生蚴成活率分析

张帆, 黄松, 姜洪杰

首都医科大学寄生虫学教研室

收稿日期:2001-12-01 修回日期:1900-01-01 出版日期:2002-07-15 发布日期:2002-07-15

Analysis on Survival Rate in Different Kinds of Culture Medium for Newborn Larvae of Trichinella spiralis

Zhang Fan, Huang Song, Jiang Hongjie

Department of Parasitology, Capital University of Medical Sciences

Received:2001-12-01 Revised:1900-01-01 Online:2002-07-15 Published:2002-07-15

摘要/Abstract

摘要： 用4种不同培养基对旋毛虫新生蚴进行体外连续培养,观察新生蚴存活率.结果:新生蚴在生理盐水中4h之内全部死亡;在纯199培养基中存活9d;在加有小牛血清或胎牛血清的199培养基中可存活26d.提示:新生蚴的存活率在无血清与加有血清的培养基中有显着性差异,在2种加有不同血清的培养基中无显着性差异.

关键词: 旋毛虫新生蚴, 培养基, 存活率

Abstract: Four different kinds of culture medium were used to continuously culture newborn larvae of Trichinella spiralis(T. S). The result showed that newborn larvae were dead in no more than 4 h in physiological saline, they could survive for 9 d in pure 199 culture medium, they could survive fore 26 d in 199 culture medium added with new bovine serum or fetal bovine serum. Analysis showed that there were great difference in the survival rate between the pure 199 culture medium and that with added serum, there was no significant difference in survival rate between the two culture medium added different serum.

Key words: newborn larvae of Trichinella spiralis(T.S), culture medium, survival rate

中图分类号:

R383.1⁺5

张帆;黄松;姜洪杰. 不同培养基体外培养旋毛虫新生蚴成活率分析[J]. 首都医科大学学报, 2002, 23(3): 206-207.

Zhang Fan;Huang Song;Jiang Hongjie . Analysis on Survival Rate in Different Kinds of Culture Medium for Newborn Larvae of Trichinella spiralis[J]. Journal of Capital Medical University, 2002, 23(3): 206-207.

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