关键词: 脑缺血再灌注, 血小板膜糖蛋白, 血小板活化

Abstract: Cerebral ischemia-reperfusion models were established by clamping both common carotid arteries in mice. With the flow cytometry, the expressions of CD₃₁,CD₆₁ and CD_62p on platelet membrane in model mice were measured at the 3rd h, 6th h, 12th h, 24th h, 48th h, 96th h after reperfusion. The expression of CD₃₁ on platelet membrane in mice increased at the 3rd h after reperfusion, reached the peak at the 24th h, and then decreased gradually, but the upregulation of CD₃₁ expression still existed at the 96th h. The expression of CD₆₁ on platelet membrane in mice increased at the 3rd h after reperfusion, lasted to the 24th h, and then returned to normal level at the 48th h. The expression of CD_62p on platelet membrane in mice increased at the 3rd h after reperfusion, reached the peak at the 12th h, and then decreased gradually, but maintained a higher level at the 96th h. The results indicate cerebral ischemia reperfusion can lead to the upregulation of CD₃₁ , CD₆₁ and CD_62p expression on platelet membrane in ischemia reperfusion mice, then may activate the platelets, and result in thrombosis.

Key words: cerebral ischemia-reperfusion, platelet membrane glycoprotein, platelet activation