内源性一氧化氮在急性胰腺炎大鼠并发肺损伤中的作用及其与氧化应激的关系

首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (1): 64-66.

内源性一氧化氮在急性胰腺炎大鼠并发肺损伤中的作用及其与氧化应激的关系

张淑文, 张在兴, 李非, 孙家邦

首都医科大学宣武医院外科

收稿日期:2002-02-27 修回日期:1900-01-01 出版日期:2003-01-15 发布日期:2003-01-15

Effect of Endogenous Nitric Oxide on Lung Injury Associated with Acute Pancreatitis in Rats and its Relationship to Oxidative Stress

Zhang Shuwen, Zhang Zaixing, Li Fei, Sun Jiabang

Department of Surgery, Xuanwu Hospital, Affiliate of Capital University of Medical Sciences

Received:2002-02-27 Revised:1900-01-01 Online:2003-01-15 Published:2003-01-15

摘要/Abstract

摘要： 为探讨内源性一氧化氮(NO)对大鼠急性坏死性胰腺炎并发肺损伤的作用及其与巯基物质的关系,以5%牛磺胆酸钠溶液胰胆管注射(1mL/kg)制成大鼠急性坏死性胰腺炎并发肺损伤模型,以工具药L-硝基精氨酸(L-NNA)为内源性NO阻断剂,观察内源性NO对肺损伤程度、肺组织内巯基物质和脂质过氧化终产物丙二醛(MDA)质量摩尔浓度的影响。结果:牛磺胆酸钠胰胆管注射可造成大鼠急性坏死性胰腺炎并发肺损伤,同时肺组织内巯基物质的质量摩尔浓度降低,MDA的质量摩尔浓度增加。以L-NNA(12.5mg/kg)阻断内源性NO后,肺损伤更加明显,同时肺组织内MDA的质量摩尔浓度也进一步增加,但对巯基物质的质量摩尔浓度没有影响。提示:内源性NO具有肺保护作用,其保护机制可能与抗氧化应激有关;巯基物质可能不参与NO的肺保护机制。

关键词: 一氧化氮, 肺损伤, 急性胰腺炎, 巯基物质, 氧自由基

Abstract: To investigate the effect of endogenous nitric oxide (NO) on lung injury in acute pancreatitis in rats and its relationship to sulfhydryl compounds and lipid peroxidation. Methods: Acute pancreatitis lung injury model in rats was induced by retrograde sodium taurocholate (5%) infusion into the pancreatobiliary duct (1 mL/kg), and NGnitro Larginine (L-NNA) was used as the inhibitor of endogenous NO. The effect of endogenous NO on lung injury, lung tissue levels of sulfhydryl compounds and malonaldehyde (MDA, the end product of lipid peroxidation), were evaluated respectively. Results: Sodium taurocholate administration produced evident lung injury associated with acute necrosis pancreatitis in rats, a decrease of sulfhydryl compounds and a increase of MDA in lung tissue. Pretreatment with the NO inhibitor, L-NNA(12.5 mg/kg), significantly intensified lung injury, and resulted in a further increase of lung tissue MDA, but it had no effect on the lung tissue sulfhydryl compounds. Conclusion: Endogenous NO has the effect of lung protection, and its antioxidation may be responsible, at least in part, for the protective mechanisms. Sulfhydryl compound may not be involved in NO lung protection mechanisms.

Key words: nitric oxide, lung injury, acute pancreatitis, sulfhydrylcompound, oxygen free radicals

中图分类号:

R657.5⁺1

张淑文;张在兴;李非;孙家邦. 内源性一氧化氮在急性胰腺炎大鼠并发肺损伤中的作用及其与氧化应激的关系[J]. 首都医科大学学报, 2003, 24(1): 64-66.

Zhang Shuwen;Zhang Zaixing;Li Fei;Sun Jiabang. Effect of Endogenous Nitric Oxide on Lung Injury Associated with Acute Pancreatitis in Rats and its Relationship to Oxidative Stress[J]. Journal of Capital Medical University, 2003, 24(1): 64-66.

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