链球菌组蛋白样蛋白对小鼠血清IL-6质量浓度的影响

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (1): 9-11.

链球菌组蛋白样蛋白对小鼠血清IL-6质量浓度的影响

张力平¹, 靖学芳¹, 张海燕², 平国玲¹, 沈海中¹, 李卫红², 张红春¹, 李郁英¹

1. 首都医科大学免疫学系;2. 首都医科大学细胞生物系

收稿日期:2003-06-23 修回日期:1900-01-01 出版日期:2004-01-15 发布日期:2004-01-15

Effect of Streptococcal Histone-Like Protein on IL-6 Level in Murine Serum

Zhang Liping¹, Jing Xuefang¹, Zhang Haiyan², Ping Guoling¹, Shen Haizhong¹, Li W eihong², Zhang Hongchun¹, Li Yuying¹

1. Department of Immunology, Capital University of Medical Sciences;2. Department of Microbiology, Gapital University of Medical Sciences

Received:2003-06-23 Revised:1900-01-01 Online:2004-01-15 Published:2004-01-15

摘要/Abstract

摘要： 为观察链球菌组蛋白样蛋白(HlpA)对小鼠血清白细胞介素6(IL-6)质量浓度的影响,经小鼠尾静脉注射HlpA,用7TD1IL-6依赖株的细胞存活率测定小鼠血清IL-6质量浓度。发现:链球菌HlpA(0～300μg/只)以剂量和时间依赖方式促进小鼠血清IL-6升高(P<0.05～P<0.01)。脂磷壁酸(LTA)和HlpA有协同增强作用;抗HlpA和肝素则对HlpA起抑制作用。提示:纯化的链球菌HlpA刺激小鼠血细胞过量产生IL-6,LTA对此有增强作用,抗HlpA和肝素则有抑制作用.

关键词: 链球菌, BALB/C小鼠, 白细胞介素6

Abstract: The goal was to evaluate the effect of Streptococcal HlpA on IL-6 level in muri ne serum. Serum levels of IL-6 production were measured by means of 7TD1 IL-6 dependent cell line. The IL-6 levels in murine serum induced with Streptococcal HlpA were time and concentration dependent in vivo. There was a synergistic res ponse on the IL-6 level in murine serum induced by the mixture of HlpA and LTA, compared to HlpA alone. On the contrary, anti-HlpA and heparin could restrain serum levels of IL-6 production induced by intravenous injection o f HlpA. It means that Streptococcal HlpA can increase serum levels of IL-6 pr oduction induced by intravenous injection of HlpA. There was significant synergi sm on it in mice vivo after HlpA and LTA were injected together.

Key words: Streptococcus, BALB/C mice, inter lukin-6

中图分类号:

R378.1⁺2

张力平;靖学芳;张海燕;平国玲;沈海中;李卫红;张红春;李郁英. 链球菌组蛋白样蛋白对小鼠血清IL-6质量浓度的影响[J]. 首都医科大学学报, 2004, 25(1): 9-11.

Zhang Liping;Jing Xuefang;Zhang Haiyan;Ping Guoling;Shen Haizhong;Li W eihong;Zhang Hongchun;Li Yuying. Effect of Streptococcal Histone-Like Protein on IL-6 Level in Murine Serum[J]. Journal of Capital Medical University, 2004, 25(1): 9-11.

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